H B Cai1, C C Chen, X H Ding. 1. Department of Gynecologic Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors and Hubei Cancer Clinical Study Center, Wuhan 430071, PR China. chb2105@163.com
Abstract
OBJECTIVE: To identify genetic variants in the E6 gene of HPV 16 and to examine the association between these variants and cervical cancer development. METHODS: Cervical samples were collected from 72 patients with cervical cancer and 72 age-matched control subjects, all from Hubei Province, China. HPV 16 sequence was detected with E6 gene-specific polymerase chain reaction, and variants were identified by DNA sequencing. RESULTS: HPV 16 was detected in 81% of the patients and 43% of control subjects, respectively. The rank orders of incidence of HPV 16 prototype and E6 variants were as follows: D25E (62%), the prototype (17%), E113D (9%), L83V (6%) in case subjects; the prototype (71%), D25E (23%), L83V/E113D (7%) in control subjects. The odds ratio of HPV 16 E6 variant was higher than that of prototype (RR=11.73, 95% CI=4.18-32.94, P=0.0001). CONCLUSION: The D25 E variant is the most prevalent E6 genomic variant in Hubei, China. The presence of HPV 16 E6 variant in cervical intraepithelial neoplasia (CIN) lesions may serve as a useful predictor of clinical outcome of the disease. Crown Copyright (c) 2009. Published by Elsevier Ltd. All rights reserved.
OBJECTIVE: To identify genetic variants in the E6 gene of HPV 16 and to examine the association between these variants and cervical cancer development. METHODS: Cervical samples were collected from 72 patients with cervical cancer and 72 age-matched control subjects, all from Hubei Province, China. HPV 16 sequence was detected with E6 gene-specific polymerase chain reaction, and variants were identified by DNA sequencing. RESULTS:HPV 16 was detected in 81% of the patients and 43% of control subjects, respectively. The rank orders of incidence of HPV 16 prototype and E6 variants were as follows: D25E (62%), the prototype (17%), E113D (9%), L83V (6%) in case subjects; the prototype (71%), D25E (23%), L83V/E113D (7%) in control subjects. The odds ratio of HPV 16 E6 variant was higher than that of prototype (RR=11.73, 95% CI=4.18-32.94, P=0.0001). CONCLUSION: The D25 E variant is the most prevalent E6 genomic variant in Hubei, China. The presence of HPV 16 E6 variant in cervical intraepithelial neoplasia (CIN) lesions may serve as a useful predictor of clinical outcome of the disease. Crown Copyright (c) 2009. Published by Elsevier Ltd. All rights reserved.
Authors: Juan Du; Cecilia Nordfors; Anders Näsman; Michal Sobkowiak; Mircea Romanitan; Tina Dalianis; Torbjörn Ramqvist Journal: PLoS One Date: 2012-04-27 Impact factor: 3.240
Authors: Min Sun; Lei Gao; Ying Liu; Yiqiang Zhao; Xueqian Wang; Yaqi Pan; Tao Ning; Hong Cai; Haijun Yang; Weiwei Zhai; Yang Ke Journal: PLoS One Date: 2012-05-04 Impact factor: 3.240