| Literature DB >> 19698715 |
Michel V de Oliveira1, Talita M Marin, Carolina F Clemente, Ana Paula Dalla Costa, Carla C Judice, Kleber G Franchini.
Abstract
Transient dephosphorylation of FAK at Tyr-397 is required for cell cycle withdrawal early on during myogenesis. Here, we show that upon serum starvation of C2C12 myoblasts, FAK is transiently dephosphorylated in parallel with SHP-2 activation and association with FAK. SHP-2 knockdown by RNA interference suppressed the transient upregulation of SHP-2 and dephosphorylation of FAK during myogenesis. Furthermore, depletion of SHP-2 retarded the cell cycle withdrawal and the differentiation of serum-starved myoblasts into myotubes. These data provide a mechanistic basis for the reduction in FAK activity in differentiating myoblasts, indicating that myogenesis is critically triggered by FAK/SHP-2 complex.Entities:
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Year: 2009 PMID： 19698715 DOI： 10.1016/j.febslet.2009.08.022
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124