Yufu Ye1, Lin Zhou, Xiaojun Xie, Guoping Jiang, Haiyang Xie, Shusen Zheng. 1. Key Lab of Combined Multi-organ Transplantation, Ministry of Public Health, Department of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
Abstract
BACKGROUND AND OBJECTIVES: The B7-H1/PD-1 pathway has recently been found to contribute to immune evasion of cancer cells from host immune system. This study aimed to investigate the expression of B7-H1 and its receptor PD-1 and to explore their significance in the progression of intraheptic cholangiocarcinoma (ICC). METHODS: Thirty-one surgically resected ICC tissues and the corresponding cancer adjacent tissues were enrolled from 2006 to 2007. Immunohistochemical studies were performed with antibody of B7-H1, PD-1, CD8, and CD4. Apoptosis status of tumor-infiltrating lymphocytes (TILs) was detected by TUNEL assay. RESULTS: Expression of B7-H1 and PD-1 was found to be up-regulated in ICC tissues compared with the cancer adjacent tissues. Tumor-related B7-H1 expression was significantly correlated with both tumor differentiation and pTNM stage and was inversely correlated with CD8+ TILs but not CD4+ TILs. TILs in primary carcinoma showed a high level of apoptosis. CONCLUSION: B7-H1/PD-1 pathway may be linked to malignant potential of ICC and contribute to tumor immune evasion by promoting CD8+ TILs apoptosis. Thus, this pathway may indeed be a potential therapeutic target in the treatment of this disease.
BACKGROUND AND OBJECTIVES: The B7-H1/PD-1 pathway has recently been found to contribute to immune evasion of cancer cells from host immune system. This study aimed to investigate the expression of B7-H1 and its receptor PD-1 and to explore their significance in the progression of intraheptic cholangiocarcinoma (ICC). METHODS: Thirty-one surgically resected ICC tissues and the corresponding cancer adjacent tissues were enrolled from 2006 to 2007. Immunohistochemical studies were performed with antibody of B7-H1, PD-1, CD8, and CD4. Apoptosis status of tumor-infiltrating lymphocytes (TILs) was detected by TUNEL assay. RESULTS: Expression of B7-H1 and PD-1 was found to be up-regulated in ICC tissues compared with the cancer adjacent tissues. Tumor-related B7-H1 expression was significantly correlated with both tumor differentiation and pTNM stage and was inversely correlated with CD8+ TILs but not CD4+ TILs. TILs in primary carcinoma showed a high level of apoptosis. CONCLUSION: B7-H1/PD-1 pathway may be linked to malignant potential of ICC and contribute to tumor immune evasion by promoting CD8+ TILs apoptosis. Thus, this pathway may indeed be a potential therapeutic target in the treatment of this disease.
Authors: Francesco Sabbatino; Vincenzo Villani; Jennifer H Yearley; Vikram Deshpande; Lei Cai; Ioannis T Konstantinidis; Christina Moon; Sjoerd Nota; Yangyang Wang; Ahmad Al-Sukaini; Andrew X Zhu; Lipika Goyal; David T Ting; Nabeel Bardeesy; Theodore S Hong; Carlos Fernandez-del Castillo; Kenneth K Tanabe; Keith D Lillemoe; Soldano Ferrone; Cristina R Ferrone Journal: Clin Cancer Res Date: 2015-09-15 Impact factor: 12.531
Authors: Jason I Griffiths; Pierre Wallet; Lance T Pflieger; David Stenehjem; Xuan Liu; Patrick A Cosgrove; Neena A Leggett; Jasmine A McQuerry; Gajendra Shrestha; Maura Rossetti; Gemalene Sunga; Philip J Moos; Frederick R Adler; Jeffrey T Chang; Sunil Sharma; Andrea H Bild Journal: Proc Natl Acad Sci U S A Date: 2020-06-22 Impact factor: 11.205