| Literature DB >> 19695656 |
Ashika Singh1, Taryn Page, Penny L Moore, Rachel L Allgaier, Keshni Hiramen, Hoosen M Coovadia, Bruce D Walker, Lynn Morris, Thumbi Ndung'u.
Abstract
It is widely documented that a complete switch from the predominant CCR5 (R5) to CXCR4 (X4) phenotype is less common for HIV-1 subtype C (HIV-1C) compared to other major subtypes. We investigated whether dualtropic HIV-1C isolates represented dualtropic, mixed R5 and X4 clones or both. Thirty of 35 functional HIV-1 env clones generated by bulk PCR amplification from peripheral blood mononuclear cells (PBMCs) infected with seven dualtropic HIV-1C isolates utilized CXCR4 exclusively. Five of 35 clones displayed dualtropism. Endpoint dilution of one isolate did not yield a substantial proportion of R5-monotropic env clones. Sequence-based predictive algorithms showed that env sequences from PBMCs, CXCR4 or CCR5-expressing cell lines were indistinguishable and all possessed X4/dualtropic characteristics. We describe HIV-1C CXCR4-tropic env sequence features. Our results suggest a dramatic loss of CCR5 monotropism as dualtropism emerges in HIV-1C which has important implications for the use of coreceptor antagonists in therapeutic strategies for this subtype.Entities:
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Year: 2009 PMID： 19695656 PMCID： PMC3492694 DOI： 10.1016/j.virol.2009.07.021
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616