Literature DB >> 19694768

Differentiation of Cryptococcus neoformans varieties and Cryptococcus gattii using CAP59-based loop-mediated isothermal DNA amplification.

S Lucas1, M da Luz Martins, O Flores, W Meyer, I Spencer-Martins, J Inácio.   

Abstract

Members of the Cryptococcus species complex (C. neoformans and C. gattii) are opportunistic pathogens responsible for frequently fatal cases of meningoencephalitis. These yeasts have been classified into five serotypes. Serotypes A and D are assigned to C. neoformans var. grubii and C. neoformans var. neoformans, respectively, Serotype AD strains are hybrids and serotype B and C strains are considered to belong to the related but distinct species C. gattii. Previous studies have identified 'serotype-associated' alleles of several genes in the Cryptococcus species complex. We developed a loop-mediated isothermal DNA amplification method using CAP59 allele-specific primers to identify the serotypes A, D and B/C of the Cryptococcus species complex.

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Year:  2009        PMID： 19694768     DOI： 10.1111/j.1469-0691.2009.02919.x

Source DB:  PubMed          Journal:  Clin Microbiol Infect        ISSN： 1198-743X            Impact factor:   8.067


  8 in total

1.  Development of a singleplex PCR assay for rapid identification and differentiation of Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and hybrids.

Authors:  Xiaobo Feng; Xiaohua Fu; Bo Ling; Lei Wang; Wanqing Liao; Zhirong Yao
Journal:  J Clin Microbiol       Date:  2013-03-27       Impact factor: 5.948

2.  Insights on the genotype distribution among Cryptococcus neoformans and C. gattii Portuguese clinical isolates.

Authors:  A P Maduro; K Mansinho; F Teles; I Silva; W Meyer; M L Martins; J Inácio
Journal:  Curr Microbiol       Date:  2014-02       Impact factor: 2.188

3.  HIV/AIDS-Associated Cryptococcosis in Portugal Spanning the Pre- to Post-HAART Era: A Retrospective Assessment at the Genotypic Level Based on URA5-RFLP.

Authors:  A P Maduro; L Gonçalves; J Inácio; N C G Faria; M L Martins; F R R Teles
Journal:  Curr Microbiol       Date:  2015-07-14       Impact factor: 2.188

4.  Matrix-assisted laser desorption ionization-time of flight mass spectrometry-based method for discrimination between molecular types of Cryptococcus neoformans and Cryptococcus gattii.

Authors:  Brunella Posteraro; Antonietta Vella; Massimo Cogliati; Elena De Carolis; Ada Rita Florio; Patrizia Posteraro; Maurizio Sanguinetti; Anna Maria Tortorano
Journal:  J Clin Microbiol       Date:  2012-05-09       Impact factor: 5.948

5.  Disseminated cryptococcosis in an apparently immunocompetent patient presenting with primary intraventricular haemorrhage.

Authors:  Partha Pal; Sayantan Ray; Sisir Kumar Patra; Debabrata Mukherjee
Journal:  BMJ Case Rep       Date:  2015-10-22

6.  Epidemiology of invasive fungal infections in the mediterranean area.

Authors:  Ulrike Binder; Cornelia Lass-Flörl
Journal:  Mediterr J Hematol Infect Dis       Date:  2011-03-31       Impact factor: 2.576

7.  Molecular techniques for pathogen identification and fungus detection in the environment.

Authors:  Clement K M Tsui; James Woodhall; Wen Chen; C André Lévesque; Anna Lau; Cor D Schoen; Christiane Baschien; Mohammad J Najafzadeh; G Sybren de Hoog
Journal:  IMA Fungus       Date:  2011-11-18       Impact factor: 3.515

8.  Identification of the major molecular types of Cryptococcus neoformans and C. gattii by Hyperbranched rolling circle amplification.

Authors:  Luciana Trilles; Bin Wang; Carolina Firacative; Márcia Dos Santos Lazéra; Bodo Wanke; Wieland Meyer
Journal:  PLoS One       Date:  2014-04-15       Impact factor: 3.240
  8 in total

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