Xian-qi ZHANG1, Min ZHENG, Kuan-hou MOU, Jie FENG. 1. Department of Dermatology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
Abstract
OBJECTIVE: To investigate the effects of 8-methoxypsoralen on human melanocytes [Ca(2+)]i and cytoskeleton actin organization in vitro. METHODS: Human melanocytes were obtained from normal foreskins. Laser confocal microscope was employed to measure [Ca(2+)]i and rhodamine-conjugated phalloidin was used to visualize the cytoskeleton actin. RESULTS: 8-methoxypsoralen increased [Ca(2+)]i and induced organization of actin stress fiber cytoskeleton. CONCLUSION: 8-methoxypsoralen might influence the migration of melanocytes by increasing the intracellular free Ca(2+) concentration and cytoskeleton actin reorganization.
OBJECTIVE: To investigate the effects of 8-methoxypsoralen on human melanocytes [Ca(2+)]i and cytoskeleton actin organization in vitro. METHODS:Human melanocytes were obtained from normal foreskins. Laser confocal microscope was employed to measure [Ca(2+)]i and rhodamine-conjugated phalloidin was used to visualize the cytoskeleton actin. RESULTS:8-methoxypsoralen increased [Ca(2+)]i and induced organization of actin stress fiber cytoskeleton. CONCLUSION:8-methoxypsoralen might influence the migration of melanocytes by increasing the intracellular free Ca(2+) concentration and cytoskeleton actin reorganization.
Authors: C Dou; Y Chen; N Ding; N Li; H Jiang; C Zhao; F Kang; Z Cao; H Quan; F Luo; J Xu; S Dong Journal: Osteoporos Int Date: 2016-01-25 Impact factor: 4.507