AIMS: This research evaluated the utilization of a urease in-house test, culture and molecular method (ureA PCR) as a diagnostic tool for Helicobacter pylori infection. Furthermore, we assessed the presence of the cagA gene in the specimens and in isolated strains that were positive for ureA by PCR positive. RESULTS: Sensitivity and specificity, respectively, were 100 and 95.8% for the urease in-house test 93.3 and 95.8 for the ureA PCR assay of the specimen and 100 and 100% for the culture. The presence of the cagA gene was observed in eight (53%) ureA-positive samples. CONCLUSIONS: In this study, we found that the PCR technique has applicability in the study of cagA, and other genes related to the H. pylori pathogen. This method can be applied to samples directly from biopsy or isolated from the bacteria.
AIMS: This research evaluated the utilization of a urease in-house test, culture and molecular method (ureA PCR) as a diagnostic tool for Helicobacter pylori infection. Furthermore, we assessed the presence of the cagA gene in the specimens and in isolated strains that were positive for ureA by PCR positive. RESULTS: Sensitivity and specificity, respectively, were 100 and 95.8% for the urease in-house test 93.3 and 95.8 for the ureA PCR assay of the specimen and 100 and 100% for the culture. The presence of the cagA gene was observed in eight (53%) ureA-positive samples. CONCLUSIONS: In this study, we found that the PCR technique has applicability in the study of cagA, and other genes related to the H. pylori pathogen. This method can be applied to samples directly from biopsy or isolated from the bacteria.
Authors: R Fabre; I Sobhani; P Laurent-Puig; N Hedef; N Yazigi; C Vissuzaine; I Rodde; F Potet; M Mignon; J P Etienne Journal: Gut Date: 1994-07 Impact factor: 23.059
Authors: Woo Chul Chung; Sung Hoon Jung; Jung Hwan Oh; Tae Ho Kim; Dae Young Cheung; Byung Wook Kim; Sung Soo Kim; Jin Il Kim; Eun Young Sin Journal: World J Gastroenterol Date: 2014-06-07 Impact factor: 5.742