Literature DB >> 19691144

Mitochondrial malic enzyme (ME2) in pancreatic islets of the human, rat and mouse and clonal insulinoma cells.

Michael J MacDonald1, Melissa J Longacre, Mindy A Kendrick.   

Abstract

Despite interest in malic enzyme(ME)s in insulin cells, mitochondrial malic enzyme (ME2) has only been studied with estimates of mRNA or with mRNA knockdown. Because an mRNA's level does not necessarily reflect the level of its cognate enzyme, we designed a simple spectrophotometric enzyme assay to measure ME2 activity of insulin cells by utilizing the distinct kinetic properties of ME2. Mitochondrial ME2 uses either NAD or NADP as a cofactor, has a high Km for malate and is allosterically activated by fumarate and inhibited by ATP. Cytosolic ME (ME1) and the other mitochondrial ME (ME3) use only NADP as a cofactor and have lower Kms for malate. The assay easily showed for the first time that substantial ME2 activity is present in pancreatic islets of humans, rats and mice and INS-1 832/13 cells. ME2's presence was confirmed with immunoblotting. There was no evidence that ME3 is present in these tissues.

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Year:  2009        PMID： 19691144      PMCID： PMC3153071          DOI： 10.1016/j.abb.2009.07.004

Source DB:  PubMed          Journal:  Arch Biochem Biophys        ISSN： 0003-9861            Impact factor:   4.013


  32 in total

1.  Malic enzymes of rabbit heart mitochondria. Separation and comparison of some characteristics of a nicotinamide adenine dinucleotide-preferring and a nicotinamide adenine dinucleotide phosphate-specific enzyme.

Authors:  R C Lin; E J Davis
Journal:  J Biol Chem       Date:  1974-06-25       Impact factor: 5.157

2.  The mitochondrial malic enzymes. I. Submitochondrial localization and purification and properties of the NAD(P)+-dependent enzyme from adrenal cortex.

Authors:  R D Mandella; L A Sauer
Journal:  J Biol Chem       Date:  1975-08-10       Impact factor: 5.157

3.  Survey of normal appearing mouse strain which lacks malic enzyme and Nad+-linked glycerol phosphate dehydrogenase: normal pancreatic beta cell function, but abnormal metabolite pattern in skeletal muscle.

Authors:  M J MacDonald; L K Marshall
Journal:  Mol Cell Biochem       Date:  2001-04       Impact factor: 3.396

4.  Structure and function of malic enzymes, a new class of oxidative decarboxylases.

Authors:  Gu-Gang Chang; Liang Tong
Journal:  Biochemistry       Date:  2003-11-11       Impact factor: 3.162

5.  Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion.

Authors:  Emma Heart; Gary W Cline; Leon P Collis; Rebecca L Pongratz; Joshua P Gray; Peter J S Smith
Journal:  Am J Physiol Endocrinol Metab       Date:  2009-03-17       Impact factor: 4.310

6.  The redox state of free nicotinamide-adenine dinucleotide phosphate in the cytoplasm of rat liver.

Authors:  R L Veech; L V Eggleston; H A Krebs
Journal:  Biochem J       Date:  1969-12       Impact factor: 3.857

7.  Enzymes of glucose metabolism in normal mouse pancreatic islets.

Authors:  S J Ashcroft; P J Randle
Journal:  Biochem J       Date:  1970-08       Impact factor: 3.857

8.  Differences between mouse and rat pancreatic islets: succinate responsiveness, malic enzyme, and anaplerosis.

Authors:  Michael J MacDonald
Journal:  Am J Physiol Endocrinol Metab       Date:  2002-08       Impact factor: 4.310

9.  13C NMR isotopomer analysis of anaplerotic pathways in INS-1 cells.

Authors:  Gary W Cline; Rebecca L Lepine; Klearchos K Papas; Richard G Kibbey; Gerald I Shulman
Journal:  J Biol Chem       Date:  2004-08-09       Impact factor: 5.157

10.  Identification and biochemical analysis of mouse mutants deficient in cytoplasmic malic enzyme.

Authors:  C Y Lee; S M Lee; S Lewis; F M Johnson
Journal:  Biochemistry       Date:  1980-10-28       Impact factor: 3.162
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  9 in total

1.  Cellular and molecular remodeling of inguinal adipose tissue mitochondria by dietary methionine restriction.

Authors:  Yuvraj N Patil; Kelly N Dille; David H Burk; Cory C Cortez; Thomas W Gettys
Journal:  J Nutr Biochem       Date:  2015-07-22       Impact factor: 6.048

Review 2.  Regulatory role of adenosine in insulin secretion from pancreatic β-cells--action via adenosine A₁ receptor and beyond.

Authors:  Tomasz Szkudelski; Katarzyna Szkudelska
Journal:  J Physiol Biochem       Date:  2014-11-30       Impact factor: 4.158

3.  Differences between human and rodent pancreatic islets: low pyruvate carboxylase, atp citrate lyase, and pyruvate carboxylation and high glucose-stimulated acetoacetate in human pancreatic islets.

Authors:  Michael J MacDonald; Melissa J Longacre; Scott W Stoker; Mindy Kendrick; Ansaya Thonpho; Laura J Brown; Noaman M Hasan; Sarawut Jitrapakdee; Toshiyuki Fukao; Matthew S Hanson; Luis A Fernandez; Jon Odorico
Journal:  J Biol Chem       Date:  2011-03-22       Impact factor: 5.157

4.  Lower succinyl-CoA:3-ketoacid-CoA transferase (SCOT) and ATP citrate lyase in pancreatic islets of a rat model of type 2 diabetes: knockdown of SCOT inhibits insulin release in rat insulinoma cells.

Authors:  Noaman M Hasan; Melissa J Longacre; Mohammed Seed Ahmed; Mindy A Kendrick; Harvest Gu; Claes-Goran Ostenson; Toshiyuki Fukao; Michael J MacDonald
Journal:  Arch Biochem Biophys       Date:  2010-05-09       Impact factor: 4.013

5.  Mitochondrial malic enzyme 3 is important for insulin secretion in pancreatic β-cells.

Authors:  Noaman M Hasan; Melissa J Longacre; Scott W Stoker; Mindy A Kendrick; Michael J MacDonald
Journal:  Mol Endocrinol       Date:  2015-01-16

Review 6.  Metabolic cycles and signals for insulin secretion.

Authors:  Matthew J Merrins; Barbara E Corkey; Richard G Kibbey; Marc Prentki
Journal:  Cell Metab       Date:  2022-06-20       Impact factor: 31.373

Review 7.  Mechanisms of amino acid-stimulated insulin secretion in congenital hyperinsulinism.

Authors:  Tingting Zhang; Changhong Li
Journal:  Acta Biochim Biophys Sin (Shanghai)       Date:  2012-12-04       Impact factor: 3.848

8.  Chronic reduction of the cytosolic or mitochondrial NAD(P)-malic enzyme does not affect insulin secretion in a rat insulinoma cell line.

Authors:  Laura J Brown; Melissa J Longacre; Noaman M Hasan; Mindy A Kendrick; Scott W Stoker; Michael J Macdonald
Journal:  J Biol Chem       Date:  2009-12-18       Impact factor: 5.157

9.  Integrated In Vivo Quantitative Proteomics and Nutrient Tracing Reveals Age-Related Metabolic Rewiring of Pancreatic β Cell Function.

Authors:  Matthew Wortham; Jacqueline R Benthuysen; Martina Wallace; Jeffrey N Savas; Francesca Mulas; Ajit S Divakaruni; Fenfen Liu; Verena Albert; Brandon L Taylor; Yinghui Sui; Enrique Saez; Anne N Murphy; John R Yates; Christian M Metallo; Maike Sander
Journal:  Cell Rep       Date:  2018-12-04       Impact factor: 9.423
  9 in total

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