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Literature DB >> 19690947

On the resolution capabilities and limits of fluorescence lifetime correlation spectroscopy (FLCS) measurements.

Steffen Rüttinger¹, Peter Kapusta, Matthias Patting, Michael Wahl, Rainer Macdonald.

Abstract

Quantitative tests were performed in order to explore the practical limits of FLCS. We demonstrate that: a) FLCS yields precise and correct concentration values from as low as picomolar to micromolar concentrations; b) it is possible to separate four signal components in a single detector setup; c) diffusion times differing only 25% from each other can be resolved by separating a two component mixture based on the different fluorescence lifetimes of both components; d) most of the inherent technical limitations of conventional FCS are easily overcome by FLCS employing a single detector channel confocal detection scheme.

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Year: 2009 PMID： 19690947 DOI： 10.1007/s10895-009-0528-1

Source DB: PubMed Journal: J Fluoresc ISSN： 1053-0509 Impact factor: 2.217

25 in total

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Authors: Tobias Kohl; Katrin G Heinze; Rene Kuhlemann; Andre Koltermann; Petra Schwille
Journal: Proc Natl Acad Sci U S A Date: 2002-09-03 Impact factor: 11.205

2. Time-resolved confocal fluorescence imaging and spectrocopy system with single molecule sensitivity and sub-micrometer resolution.

Authors: M Wahl; F Koberling; M Patting; H Rahn; R Erdmann
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3. Time-resolved methods in biophysics. 3. Fluorescence lifetime correlation spectroscopy.

Authors: Ingo Gregor; Jörg Enderlein
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4. Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements.

Authors: Thomas Dertinger; Victor Pacheco; Iris von der Hocht; Rudolf Hartmann; Ingo Gregor; Jörg Enderlein
Journal: Chemphyschem Date: 2007-02-19 Impact factor: 3.102

5. Dead-time optimized time-correlated photon counting instrument with synchronized, independent timing channels.

Authors: Michael Wahl; Hans-Jürgen Rahn; Ingo Gregor; Rainer Erdmann; Jörg Enderlein
Journal: Rev Sci Instrum Date: 2007-03 Impact factor: 1.523

6. Confocal fluorescence coincidence analysis: an approach to ultra high-throughput screening.

Authors: T Winkler; U Kettling; A Koltermann; M Eigen
Journal: Proc Natl Acad Sci U S A Date: 1999-02-16 Impact factor: 11.205

7. Fluorescence cross-correlation: a new concept for polymerase chain reaction.

Authors: R Rigler; Z Földes-Papp; F J Meyer-Almes; C Sammet; M Völcker; A Schnetz
Journal: J Biotechnol Date: 1998-08-12 Impact factor: 3.307

8. Rapid assay processing by integration of dual-color fluorescence cross-correlation spectroscopy: high throughput screening for enzyme activity.

Authors: A Koltermann; U Kettling; J Bieschke; T Winkler; M Eigen
Journal: Proc Natl Acad Sci U S A Date: 1998-02-17 Impact factor: 11.205

9. Ultrasensitive detection of pathological prion protein aggregates by dual-color scanning for intensely fluorescent targets.

Authors: J Bieschke; A Giese; W Schulz-Schaeffer; I Zerr; S Poser; M Eigen; H Kretzschmar
Journal: Proc Natl Acad Sci U S A Date: 2000-05-09 Impact factor: 11.205

On the resolution capabilities and limits of fluorescence lifetime correlation spectroscopy (FLCS) measurements.

1. A protease assay for two-photon crosscorrelation and FRET analysis based solely on fluorescent proteins.

2. Time-resolved confocal fluorescence imaging and spectrocopy system with single molecule sensitivity and sub-micrometer resolution.

3. Time-resolved methods in biophysics. 3. Fluorescence lifetime correlation spectroscopy.

4. Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements.

5. Dead-time optimized time-correlated photon counting instrument with synchronized, independent timing channels.

6. Confocal fluorescence coincidence analysis: an approach to ultra high-throughput screening.

7. Fluorescence cross-correlation: a new concept for polymerase chain reaction.

8. Rapid assay processing by integration of dual-color fluorescence cross-correlation spectroscopy: high throughput screening for enzyme activity.

9. Ultrasensitive detection of pathological prion protein aggregates by dual-color scanning for intensely fluorescent targets.

10. Fluorescence correlation spectroscopy. II. An experimental realization.

1. Pulsed interleaved excitation fluctuation imaging.

2. Filtered FCS: species auto- and cross-correlation functions highlight binding and dynamics in biomolecules.

3. A Single-Strand Annealing Protein Clamps DNA to Detect and Secure Homology.

Review 4. Fluorescence Lifetime Correlation Spectroscopy (FLCS): concepts, applications and outlook.

5. Early amyloidogenic oligomerization studied through fluorescence lifetime correlation spectroscopy.