OBJECTIVES: The objectives of this study were to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) colonization in livestock and related workers in four Chinese provinces and the characteristics of these isolates. METHODS: Nasal swabs were collected from animals and farm workers in four Chinese provinces. MRSA isolates were recovered and characterized by PFGE, Panton-Valentine leucocidin PCR, staphylococcal chromosomal cassette (SCC) mec typing, spa typing, multilocus sequence typing, antimicrobial susceptibility testing and testing for inducible clindamycin resistance. RESULTS: A total of 60 MRSA isolates were recovered from swine and swine workers. Two predominant multidrug resistance profiles were identified: ciprofloxacin/clindamycin/erythromycin/cefoxitin/gentamicin/tetracycline/chloramphenicol and ciprofloxacin/clindamycin/erythromycin/cefoxitin/gentamicin/tetracycline. All isolates were determined to be spa type t899, contained the group III SCCmec element and were Panton-Valentine leucocidin negative. Multilocus sequence type ST9 (n = 46) was identified as the dominant sequence type. One dominant PFGE cluster and a dominant strain type were identified. CONCLUSIONS: MRSA from Chinese pigs and farm workers (ST9) differed from the European pig-associated clone (ST398) with regard to clonal type, SCCmec content and resistance profile.
OBJECTIVES: The objectives of this study were to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) colonization in livestock and related workers in four Chinese provinces and the characteristics of these isolates. METHODS: Nasal swabs were collected from animals and farm workers in four Chinese provinces. MRSA isolates were recovered and characterized by PFGE, Panton-Valentine leucocidin PCR, staphylococcal chromosomal cassette (SCC) mec typing, spa typing, multilocus sequence typing, antimicrobial susceptibility testing and testing for inducible clindamycin resistance. RESULTS: A total of 60 MRSA isolates were recovered from swine and swine workers. Two predominant multidrug resistance profiles were identified: ciprofloxacin/clindamycin/erythromycin/cefoxitin/gentamicin/tetracycline/chloramphenicol and ciprofloxacin/clindamycin/erythromycin/cefoxitin/gentamicin/tetracycline. All isolates were determined to be spa type t899, contained the group III SCCmec element and were Panton-Valentine leucocidin negative. Multilocus sequence type ST9 (n = 46) was identified as the dominant sequence type. One dominant PFGE cluster and a dominant strain type were identified. CONCLUSIONS: MRSA from Chinese pigs and farm workers (ST9) differed from the European pig-associated clone (ST398) with regard to clonal type, SCCmec content and resistance profile.
Authors: Kerry R Leedom Larson; Abby L Harper; Blake M Hanson; Michael J Male; Shylo E Wardyn; Anne E Dressler; Elizabeth A Wagstrom; Shaliesh Tendolkar; Daniel J Diekema; Kelley J Donham; Tara C Smith Journal: Appl Environ Microbiol Date: 2010-11-19 Impact factor: 4.792
Authors: Meghan F Davis; Nora Pisanic; Sarah M Rhodes; Alexis Brown; Haley Keller; Maya Nadimpalli; Andrea Christ; Shanna Ludwig; Carly Ordak; Kristoffer Spicer; David C Love; Jesper Larsen; Asher Wright; Sarah Blacklin; Billy Flowers; Jill Stewart; Kenneth G Sexton; Ana M Rule; Christopher D Heaney Journal: Environ Res Date: 2018-02-22 Impact factor: 6.498
Authors: L M Cavaco; H Hasman; M Stegger; P S Andersen; R Skov; A C Fluit; T Ito; F M Aarestrup Journal: Antimicrob Agents Chemother Date: 2010-06-28 Impact factor: 5.191
Authors: Vasanthakumari Neela; Arif Mohd Zafrul; Nor Shamsudin Mariana; Alex van Belkum; Yun Khoon Liew; Ehsanollah Ghaznavi Rad Journal: J Clin Microbiol Date: 2009-10-07 Impact factor: 5.948