| Literature DB >> 19682995 |
Phuong Dinh Tam1, Nguyen Van Hieu, Nguyen Duc Chien, Anh-Tuan Le, Mai Anh Tuan.
Abstract
This paper describes the DNA immobilization using carbon multi-walled nanotubes (MWCNTs) for direct and label-free detection of influenza virus (type A). The DNA probe was attached on the sensor surface by means of covalent bonding between the amine and phosphate groups of the DNA sequence. The interaction between the DNA probe and the MWCNTs were characterized by Fourier Transform Infrared (FTIR) spectrometry, Raman spectra. The hybridization of the DNA probe and the target DNA were detected by changes in the conductance on the surface of sensors leading to the change in the output signal of the system. The results show that the DNA sensor can detect as low as 0.5 nM of the target DNA samples; the response time of DNA sensor is approximately 4 min.Entities:
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Year: 2009 PMID： 19682995 DOI： 10.1016/j.jim.2009.08.002
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303