Literature DB >> 19682457

PCR-RFLP analysis of chitinase genes enables efficient genotyping of Metarhizium anisopliae var. anisopliae.

Jürg Enkerli1, Vandana Ghormade, Catherine Oulevey, Franco Widmer.   

Abstract

A new genotyping tool has been developed and evaluated for Metarhizium anisopliae var. anisopliae. The tool is based on Restriction Fragment Length Polymorphism (RFLP) analysis of three chitinase genes that are functionally linked to insect-pathogenicity of this fungus. It allowed for discrimination of 14 genotypes among 22 M. anisopliae var. anisopliae strains of a world wide collection. Analyses revealed that the approach may also be applicable to other Metarhizium varieties. The new tool will be useful for genetic characterization of M. anisopliae var. anisopliae strains, and it is applicable for laboratories with limited access to molecular diagnostic equipment.

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Year:  2009        PMID： 19682457     DOI： 10.1016/j.jip.2009.08.006

Source DB:  PubMed          Journal:  J Invertebr Pathol        ISSN： 0022-2011            Impact factor:   2.841


  5 in total

1.  Development of Metarhizium anisopliae as a Mycoinsecticide: From Isolation to Field Performance.

Authors:  Santosh G Tupe; Ejaj K Pathan; Mukund V Deshpande
Journal:  J Vis Exp       Date:  2017-07-30       Impact factor: 1.355

2.  Genetic diversity analysis reveals that geographical environment plays a more important role than rice cultivar in Villosiclava virens population selection.

Authors:  Fei Wang; Shu Zhang; Mei-Gang Liu; Xian-Song Lin; Hui-Jiang Liu; You-Liang Peng; Yang Lin; Jun-Bin Huang; Chao-Xi Luo
Journal:  Appl Environ Microbiol       Date:  2014-02-28       Impact factor: 4.792

3.  Genetic diversity of the fungal pathogen Metarhizium spp., causing epizootics in Chinese burrower bugs in the Jingting Mountains, eastern China.

Authors:  Fenggang Luan; Shengli Zhang; Bin Wang; Bo Huang; Zengzhi Li
Journal:  Mol Biol Rep       Date:  2012-10-16       Impact factor: 2.316

4.  Biodiversity of Entomopathogenic Fungi in the Soils of South China.

Authors:  Xiaoyan Niu; Weiwen Xie; Jing Zhang; Qiongbo Hu
Journal:  Microorganisms       Date:  2019-09-03

5.  Use of Metarhizium anisopliae chitinase genes for genotyping and virulence characterization.

Authors:  Saliou Niassy; Sevgan Subramanian; Sunday Ekesi; Joel L Bargul; Jandouwe Villinger; Nguya K Maniania
Journal:  Biomed Res Int       Date:  2013-07-09       Impact factor: 3.411
  5 in total

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