Literature DB >> 19680778

Purification and characterization of cytochrome c(6) from Acaryochloris marina.

Patrick D Bell1, Yueyong Xin, Robert E Blankenship.   

Abstract

Cytochrome c(6), (cyt c(6)) a soluble monoheme electron transport protein, was isolated and characterized from the chlorophyll d-containing cyanobacterium Acaryochoris marina, the type strain MBIC11017. The protein was purified using ammonium sulfate precipitation, ion exchange and gel filtration column chromatography, and fast performance liquid chromatography. Its molecular mass and pI have been determined to be 8.87 kDa and less than 4.2, respectively, by mass spectrometry and isoelectrofocusing (IEF). The protein has an alpha helical structure as indicated by CD (circular dichroism) spectroscopy and a reduction midpoint potential (E(m)) of +327 mV versus the normal hydrogen electrode (NHE) as determined by redox potentiometry. Its potential role in electron transfer processes is discussed.

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Year:  2009        PMID： 19680778      PMCID： PMC3947841          DOI： 10.1007/s11120-009-9482-7

Source DB:  PubMed          Journal:  Photosynth Res        ISSN： 0166-8595            Impact factor:   3.573


  25 in total

1.  Extinction coefficients and midpoint potentials of cytochrome c(6) from the cyanobacteria Arthrospira maxima, Microcystis aeruginosa, and Synechocystis 6803.

Authors:  Y S Cho; Q J Wang; D Krogmann; J Whitmarsh
Journal:  Biochim Biophys Acta       Date:  1999-10-06

2.  Electrocatalytic investigation of light-induced electron transfer between cytochrome c6 and photosystem I.

Authors:  Vanessa Proux-Delrouyre; Christophe Demaille; Winfried Leibl; Pierre Sétif; Hervé Bottin; Christian Bourdillon
Journal:  J Am Chem Soc       Date:  2003-11-12       Impact factor: 15.419

Review 3.  Unique photosystems in Acaryochloris marina.

Authors:  Shunsuke Ohashi; Hideaki Miyashita; Naoki Okada; Tatsuya Iemura; Tadashi Watanabe; Masami Kobayashi
Journal:  Photosynth Res       Date:  2008-11-05       Impact factor: 3.573

4.  The primary electron donor of photosystem II of the cyanobacterium Acaryochloris marina is a chlorophyll d and the water oxidation is driven by a chlorophyll a/chlorophyll d heterodimer.

Authors:  T Renger; E Schlodder
Journal:  J Phys Chem B       Date:  2008-05-30       Impact factor: 2.991

5.  A comparative laser-flash absorption spectroscopy study of Anabaena PCC 7119 plastocyanin and cytochrome c6 photooxidation by photosystem I particles.

Authors:  M Medina; A Díaz; M Hervás; J A Navarro; C Gómez-Moreno; M A de la Rosa; G Tollin
Journal:  Eur J Biochem       Date:  1993-05-01

6.  Effect of iron on growth and ultrastructure of Acaryochloris marina.

Authors:  Wesley D Swingley; Martin F Hohmann-Marriott; Tien Le Olson; Robert E Blankenship
Journal:  Appl Environ Microbiol       Date:  2005-12       Impact factor: 4.792

7.  Cytochrome c6 from Cyanophora paradoxa. Characterization of the protein and the cDNA of the precursor and import into isolated cyanelles.

Authors:  J M Steiner; A Serrano; G Allmaier; J Jakowitsch; W Löffelhardt
Journal:  Eur J Biochem       Date:  2000-07

8.  Cytochrome c6 from Monoraphidium braunii. A cytochrome with an unusual heme axial coordination.

Authors:  A P Campos; A P Aguiar; M Hervás; M Regalla; J A Navarro; J M Ortega; A V Xavier; M A De La Rosa; M Teixeira
Journal:  Eur J Biochem       Date:  1993-08-15

9.  Energetics of Photosystem II charge recombination in Acaryochloris marina studied by thermoluminescence and flash-induced chlorophyll fluorescence measurements.

Authors:  Krisztián Cser; Zsuzsanna Deák; Alison Telfer; James Barber; Imre Vass
Journal:  Photosynth Res       Date:  2008-10-07       Impact factor: 3.573

10.  Membrane orientation of the FMO antenna protein from Chlorobaculum tepidum as determined by mass spectrometry-based footprinting.

Authors:  Jianzhong Wen; Hao Zhang; Michael L Gross; Robert E Blankenship
Journal:  Proc Natl Acad Sci U S A       Date:  2009-04-01       Impact factor: 11.205
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  2 in total

1.  Structural analysis of alternative complex III in the photosynthetic electron transfer chain of Chloroflexus aurantiacus.

Authors:  Xinliu Gao; Yueyong Xin; Patrick D Bell; Jianzhong Wen; Robert E Blankenship
Journal:  Biochemistry       Date:  2010-08-10       Impact factor: 3.162

Review 2.  Chlorophyll d and Acaryochloris marina: current status.

Authors:  Patrick Loughlin; Yuankui Lin; Min Chen
Journal:  Photosynth Res       Date:  2013-04-25       Impact factor: 3.573
  2 in total

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