Literature DB >> 19679239

Real-time polymerase chain reaction detection of Cryptococcus neoformans and Cryptococcus gattii in human samples.

Vincent Veron1, Stephane Simon, Denis Blanchet, Christine Aznar.   

Abstract

We report a TaqMan real-time polymerase chain reaction (PCR) protocol for diagnosing cryptococcosis. Specificity was tested with 33 fungal strains. The 7 clinical samples found positive by culture also tested positive by real-time PCR. No false negatives were found among the 94 clinical samples that were negative by culture for Cryptococcus spp. The sensitivity threshold was about 10 plasmid copies per assay.

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Mesh：

Year:  2009        PMID： 19679239     DOI： 10.1016/j.diagmicrobio.2009.05.005

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  12 in total

1.  High-resolution melting analysis for identification of the Cryptococcus neoformans-Cryptococcus gattii complex.

Authors:  Sara Gago; Óscar Zaragoza; Isabel Cuesta; Juan L Rodríguez-Tudela; Manuel Cuenca-Estrella; María J Buitrago
Journal:  J Clin Microbiol       Date:  2011-08-10       Impact factor: 5.948

Review 2.  Cryptococcus gattii infections.

Authors:  Sharon C-A Chen; Wieland Meyer; Tania C Sorrell
Journal:  Clin Microbiol Rev       Date:  2014-10       Impact factor: 26.132

3.  A multi-target real-time PCR assay for rapid identification of meningitis-associated microorganisms.

Authors:  Marco Favaro; Vincenzo Savini; Cartesio Favalli; Carla Fontana
Journal:  Mol Biotechnol       Date:  2013-01       Impact factor: 2.695

4.  Multicenter Evaluation of BioFire FilmArray Meningitis/Encephalitis Panel for Detection of Bacteria, Viruses, and Yeast in Cerebrospinal Fluid Specimens.

Authors:  Amy L Leber; Kathy Everhart; Joan-Miquel Balada-Llasat; Jillian Cullison; Judy Daly; Sarah Holt; Paul Lephart; Hossein Salimnia; Paul C Schreckenberger; Sharon DesJarlais; Sharon L Reed; Kimberle C Chapin; Lindsay LeBlanc; J Kristie Johnson; Nicole L Soliven; Karen C Carroll; Jo-Anne Miller; Jennifer Dien Bard; Javier Mestas; Matthew Bankowski; Tori Enomoto; Andrew C Hemmert; Kevin M Bourzac
Journal:  J Clin Microbiol       Date:  2016-06-22       Impact factor: 5.948

5.  A multiplex real-time PCR assay for identification of Pneumocystis jirovecii, Histoplasma capsulatum, and Cryptococcus neoformans/Cryptococcus gattii in samples from AIDS patients with opportunistic pneumonia.

Authors:  Sara Gago; Cristina Esteban; Clara Valero; Oscar Zaragoza; Jorge Puig de la Bellacasa; María José Buitrago
Journal:  J Clin Microbiol       Date:  2014-01-29       Impact factor: 5.948

6.  Paradoxical Immune Responses in Non-HIV Cryptococcal Meningitis.

Authors:  Anil A Panackal; Simone C Wuest; Yen-Chih Lin; Tianxia Wu; Nannan Zhang; Peter Kosa; Mika Komori; Andrew Blake; Sarah K Browne; Lindsey B Rosen; Ferry Hagen; Jacques Meis; Stuart M Levitz; Martha Quezado; Dima Hammoud; John E Bennett; Bibi Bielekova; Peter R Williamson
Journal:  PLoS Pathog       Date:  2015-05-28       Impact factor: 6.823

7.  A multiplex quantitative real-time polymerase chain reaction panel for detecting neurologic pathogens in dogs with meningoencephalitis.

Authors:  Jae-Ik Han; Dong-Woo Chang; Ki-Jeong Na
Journal:  J Vet Sci       Date:  2015-06-03       Impact factor: 1.672

8.  Importance of Resolving Fungal Nomenclature: the Case of Multiple Pathogenic Species in the Cryptococcus Genus.

Authors:  Ferry Hagen; H Thorsten Lumbsch; Valentina Arsic Arsenijevic; Hamid Badali; Sebastien Bertout; R Blake Billmyre; M Rosa Bragulat; F Javier Cabañes; Mauricio Carbia; Arunaloke Chakrabarti; Sudha Chaturvedi; Vishnu Chaturvedi; Min Chen; Anuradha Chowdhary; Maria-Francisca Colom; Oliver A Cornely; Pedro W Crous; Maria S Cuétara; Mara R Diaz; Ana Espinel-Ingroff; Hamed Fakhim; Rama Falk; Wenjie Fang; Patricia F Herkert; Consuelo Ferrer Rodríguez; James A Fraser; Josepa Gené; Josep Guarro; Alexander Idnurm; María-Teresa Illnait-Zaragozi; Ziauddin Khan; Kantarawee Khayhan; Anna Kolecka; Cletus P Kurtzman; Katrien Lagrou; Wanqing Liao; Carlos Linares; Jacques F Meis; Kirsten Nielsen; Tinashe K Nyazika; Weihua Pan; Marina Pekmezovic; Itzhack Polacheck; Brunella Posteraro; Flavio de Queiroz Telles; Orazio Romeo; Manuel Sánchez; Ana Sampaio; Maurizio Sanguinetti; Pojana Sriburee; Takashi Sugita; Saad J Taj-Aldeen; Masako Takashima; John W Taylor; Bart Theelen; Rok Tomazin; Paul E Verweij; Retno Wahyuningsih; Ping Wang; Teun Boekhout
Journal:  mSphere       Date:  2017-08-30       Impact factor: 4.389

9.  Evaluation of Extraction Methods for Clinical Metagenomic Assay.

Authors:  Suha A Farraj; Shreif A El-Kafrawy; Taha A Kumosani; Jehad M Yousef; Esam I Azhar
Journal:  Microorganisms       Date:  2020-07-27

Review 10.  Application of Culture-Independent Rapid Diagnostic Tests in the Management of Invasive Candidiasis and Cryptococcosis.

Authors:  Michael A Pfaller
Journal:  J Fungi (Basel)       Date:  2015-08-31
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