Literature DB >> 19669133

Structure and heterogeneity of gliadin: a hydrodynamic evaluation.

Shirley Ang1, Jana Kogulanathan, Gordon A Morris, M Samil Kök, Peter R Shewry, Arthur S Tatham, Gary G Adams, Arthur J Rowe, Stephen E Harding.   

Abstract

A study of the heterogeneity and conformation in solution [in 70% (v/v) aq. ethanol] of gliadin proteins from wheat was undertaken based upon sedimentation velocity in the analytical ultracentrifuge, analysis of the distribution coefficients and ellipsoidal axial ratios assuming quasi-rigid particles, allowing for a range of plausible time-averaged hydration values. All classical fractions (alpha, gamma, omega(slow), omega(fast)) show three clearly resolved components. Based on the weight-average sedimentation coefficient for each fraction and a weight-average molecular weight from sedimentation equilibrium and/or cDNA sequence analysis, all the proteins are extended molecules with axial ratios ranging from ~10 to 30 with alpha appearing the most extended and gamma the least.

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Year:  2009        PMID： 19669133     DOI： 10.1007/s00249-009-0529-7

Source DB:  PubMed          Journal:  Eur Biophys J        ISSN： 0175-7571            Impact factor:   1.733


  17 in total

1.  Small angle X-ray scattering of wheat seed-storage proteins: alpha-, gamma- and omega-gliadins and the high molecular weight (HMW) subunits of glutenin.

Authors:  N H Thomson; M J Miles; Y Popineau; J Harries; P Shewry; A S Tatham
Journal:  Biochim Biophys Acta       Date:  1999-03-19

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Authors:  S E Harding
Journal:  Biophys Chem       Date:  2001-11-28       Impact factor: 2.352

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Journal:  Arch Biochem Biophys       Date:  1961-09       Impact factor: 4.013

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Journal:  Biochem J       Date:  1936-03       Impact factor: 3.857

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Authors:  N Wellner; P S Belton; A S Tatham
Journal:  Biochem J       Date:  1996-11-01       Impact factor: 3.857

6.  The ELLIPS suite of macromolecular conformation algorithms.

Authors:  S E Harding; J C Horton; H Cölfen
Journal:  Eur Biophys J       Date:  1997       Impact factor: 1.733

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Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

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Authors:  M R Booth; J A Ewart
Journal:  Biochim Biophys Acta       Date:  1969-05

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Authors:  P G Squire; M E Himmel
Journal:  Arch Biochem Biophys       Date:  1979-08       Impact factor: 4.013

10.  Small-angle X-ray-scattering studies of the C hordeins of barley (Hordeum vulgare).

Authors:  K J I'Anson; V J Morris; P R Shewry; A S Tatham
Journal:  Biochem J       Date:  1992-10-01       Impact factor: 3.857
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  9 in total

1.  Dietary Gluten as a Conditioning Factor of the Gut Microbiota in Celiac Disease.

Authors:  Karla A Bascuñán; Magdalena Araya; Leda Roncoroni; Luisa Doneda; Luca Elli
Journal:  Adv Nutr       Date:  2020-01-01       Impact factor: 8.701

2.  Analytical ultracentrifugation studies of oligomerization and DNA-binding of TtCarH, a Thermus thermophilus coenzyme B12-based photosensory regulator.

Authors:  Ana I Díez; Juan Manuel Ortiz-Guerrero; Alvaro Ortega; Montserrat Elías-Arnanz; S Padmanabhan; José García de la Torre
Journal:  Eur Biophys J       Date:  2013-03-20       Impact factor: 1.733

Review 3.  Gliadins from wheat grain: an overview, from primary structure to nanostructures of aggregates.

Authors:  Reiko Urade; Nobuhiro Sato; Masaaki Sugiyama
Journal:  Biophys Rev       Date:  2017-12-04

Review 4.  Celiac disease: understanding the gluten-free diet.

Authors:  Karla A Bascuñán; María Catalina Vespa; Magdalena Araya
Journal:  Eur J Nutr       Date:  2016-06-22       Impact factor: 5.614

5.  Hydrodynamic modelling of protein conformation in solution: ELLIPS and HYDRO.

Authors:  José García de la Torre; Stephen E Harding
Journal:  Biophys Rev       Date:  2013-02-20

6.  Can dietary fibre help provide safer food products for sufferers of gluten intolerance? A well-established biophysical probe may help towards providing an answer.

Authors:  M Samil Kök; Richard Gillis; Shirley Ang; David Lafond; Arthur S Tatham; Gary Adams; Stephen E Harding
Journal:  BMC Biophys       Date:  2012-05-17       Impact factor: 4.778

7.  Dynamics of liquid-liquid phase separation of wheat gliadins.

Authors:  Adeline Boire; Christian Sanchez; Marie-Hélène Morel; Minne Paul Lettinga; Paul Menut
Journal:  Sci Rep       Date:  2018-09-27       Impact factor: 4.379

8.  Characterization of gliadin, secalin and hordein fractions using analytical techniques.

Authors:  Monika Rani; Dalbir Singh Sogi; Balmeet Singh Gill
Journal:  Sci Rep       Date:  2021-11-30       Impact factor: 4.379

9.  Microcellular foams made from gliadin.

Authors:  S Quester; M Dahesh; R Strey
Journal:  Colloid Polym Sci       Date:  2014-06-28       Impact factor: 1.931
  9 in total

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