Literature DB >> 19668857

Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells.

Jeffrey K Takimoto1, Katrina L Adams, Zheng Xiang, Lei Wang.   

Abstract

Optimizing the anticodon recognition between orthogonal tRNA and synthetase significantly increased the incorporation efficiencies of various unnatural amino acids in mammalian cells, and the enhanced incorporation enabled efficient photocrosslinking of interacting proteins in mammalian cells.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19668857     DOI： 10.1039/b904228h

Source DB:  PubMed          Journal:  Mol Biosyst        ISSN： 1742-2051


  35 in total

1.  α-Helical element at the hormone-binding surface of the insulin receptor functions as a signaling element to activate its tyrosine kinase.

Authors:  Jonathan Whittaker; Linda J Whittaker; Charles T Roberts; Nelson B Phillips; Faramarz Ismail-Beigi; Michael C Lawrence; Michael A Weiss
Journal:  Proc Natl Acad Sci U S A       Date:  2012-06-26       Impact factor: 11.205

2.  Photo-cross-linkers incorporated into G-protein-coupled receptors in mammalian cells: a ligand comparison.

Authors:  Irene Coin; Marilyn H Perrin; Wylie W Vale; Lei Wang
Journal:  Angew Chem Int Ed Engl       Date:  2011-07-12       Impact factor: 15.336

3.  Esterification of an unnatural amino acid structurally deviating from canonical amino acids promotes its uptake and incorporation into proteins in mammalian cells.

Authors:  Jeffrey K Takimoto; Zheng Xiang; Ji-Yong Kang; Lei Wang
Journal:  Chembiochem       Date:  2010-11-02       Impact factor: 3.164

4.  Genetically encoding unnatural amino acids in neural stem cells and optically reporting voltage-sensitive domain changes in differentiated neurons.

Authors:  Bin Shen; Zheng Xiang; Barbara Miller; Gordon Louie; Wenyuan Wang; Joseph P Noel; Fred H Gage; Lei Wang
Journal:  Stem Cells       Date:  2011-08       Impact factor: 6.277

Review 5.  Antibody drug conjugates: design and selection of linker, payload and conjugation chemistry.

Authors:  Jessica R McCombs; Shawn C Owen
Journal:  AAPS J       Date:  2015-01-22       Impact factor: 4.009

6.  An expanded genetic code in Candida albicans to study protein-protein interactions in vivo.

Authors:  Silke Palzer; Yannick Bantel; Franziska Kazenwadel; Michael Berg; Steffen Rupp; Kai Sohn
Journal:  Eukaryot Cell       Date:  2013-03-29

7.  Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells.

Authors:  Robert Serfling; Lisa Seidel; Thore Böttke; Irene Coin
Journal:  J Vis Exp       Date:  2018-04-09       Impact factor: 1.355

8.  Expanding the genetic code of Caenorhabditis elegans using bacterial aminoacyl-tRNA synthetase/tRNA pairs.

Authors:  Angela R Parrish; Xingyu She; Zheng Xiang; Irene Coin; Zhouxin Shen; Steven P Briggs; Andrew Dillin; Lei Wang
Journal:  ACS Chem Biol       Date:  2012-05-11       Impact factor: 5.100

9.  Resurrecting the Bacterial Tyrosyl-tRNA Synthetase/tRNA Pair for Expanding the Genetic Code of Both E. coli and Eukaryotes.

Authors:  James S Italia; Christopher Latour; Chester J J Wrobel; Abhishek Chatterjee
Journal:  Cell Chem Biol       Date:  2018-08-02       Impact factor: 8.116

10.  Genetically encoded chemical probes in cells reveal the binding path of urocortin-I to CRF class B GPCR.

Authors:  Irene Coin; Vsevolod Katritch; Tingting Sun; Zheng Xiang; Fai Yiu Siu; Michael Beyermann; Raymond C Stevens; Lei Wang
Journal:  Cell       Date:  2013-11-27       Impact factor: 41.582
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.