Haladaptatus cibarius sp. nov., an extremely halophilic archaeon from seafood, and emended description of the genus Haladaptatus.

A novel, extremely halophilic archaeon, D43(T), was isolated from traditional salt-fermented seafood in Korea. The cells were Gram-negative-staining and motile. The strain grew at 15-50 degrees C, 10-30 % (w/v) NaCl and pH 6.0-8.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain D43(T) is affiliated with the family Halobacteriaceae in the domain Archaea and had 95.5 % 16S rRNA gene sequence similarity with Haladaptatus paucihalophilus DX253(T). The sequence from strain D43(T) formed a clade with those from Hap. paucihalophilus regardless of which tree-generating algorithm was used. DNA-DNA hybridization experiments showed 25.8 % relatedness between the isolate and Hap. paucihalophilus KCTC 4006(T). Major lipids were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two unidentified glycolipids. The DNA G+C content of the isolate was 56.5 mol%. On the basis of this polyphasic taxonomic study, strain D43(T) represents a novel species in the genus Haladaptatus, for which the name Haladaptatus cibarius sp. nov. is proposed. The type strain is D43(T) (=DSM 19505(T) =JCM 15962(T)).