AIM: To observe the effect of lysophosphatidic acid on cervical cancer cell line HeLa cells proliferation, adhesion, migration and apoptosis and study related signaling pathway. METHODS: After HeLa cells affected by LPA, the level of proliferation was drawing growth curve after cell couting; the sensitivity to cisplatin was evaluated by methyl thiazolyl tetrazolium (MTT) assay and flow cytometry was used to examine cell apoptosis; the potentiality of migration was evaluated by transwell chamber, then to observe the effect of Y27632, LY294002 and PD98059 on biological function of LPA. RESULTS: LPA could promote HeLa cell proliferate on time-dependent and dose-dependent relationship. MEK1 inhibitor could inhibit LPA-induced cell proliferation; LPA promoted the adhesion and migration of HeLa cells in dose-dependence; Rho inhibitor could inhibit the LPA-induced adhesion and migration; LPA could reduce the chemotherapy sensibility of cervical cancer to DDP in dose-dependence, then PI3K inhibitor could resist this effect. CONCLUSION: LPA could promote HeLa cells' proliferation, adhesion, migration and anti-apoptosis through multiple signaling pathways.
AIM: To observe the effect of lysophosphatidic acid on cervical cancer cell line HeLa cells proliferation, adhesion, migration and apoptosis and study related signaling pathway. METHODS: After HeLa cells affected by LPA, the level of proliferation was drawing growth curve after cell couting; the sensitivity to cisplatin was evaluated by methyl thiazolyl tetrazolium (MTT) assay and flow cytometry was used to examine cell apoptosis; the potentiality of migration was evaluated by transwell chamber, then to observe the effect of Y27632, LY294002 and PD98059 on biological function of LPA. RESULTS: LPA could promote HeLa cell proliferate on time-dependent and dose-dependent relationship. MEK1 inhibitor could inhibit LPA-induced cell proliferation; LPA promoted the adhesion and migration of HeLa cells in dose-dependence; Rho inhibitor could inhibit the LPA-induced adhesion and migration; LPA could reduce the chemotherapy sensibility of cervical cancer to DDP in dose-dependence, then PI3K inhibitor could resist this effect. CONCLUSION: LPA could promote HeLa cells' proliferation, adhesion, migration and anti-apoptosis through multiple signaling pathways.