| Literature DB >> 196563 |
Abstract
The subcellular location of hexose diphosphatase, phosphoenolpyruvate carboxykinase and pyruvate carboxylase in baker's yeast (Saccharomyces cerevisiae) was investigated by density gradient centrifugation of spheroplast lysates obtained by osmotic shock treatment of spheroplasts and centrifugation for 10000 g x min. On the evidence obtained from zonal separations these three enzymes of gluconeogenesis are most probably located in the soluble cytosol.Entities:
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Year: 1977 PMID: 196563 DOI: 10.1007/bf00428597
Source DB: PubMed Journal: Arch Microbiol ISSN: 0302-8933 Impact factor: 2.552