PURPOSE: 6-[(18)F]Fluoro-3,4-dihydroxy-L-phenylalanine (6-[(18)F]F-L-DOPA) is increasingly used for PET imaging of neuroendocrine tumours. In this study, we investigated the use of 6-[(18)F]F-L-DOPA to detect and to monitor the progression of medullary thyroid carcinoma (MTC) in a genetically engineered mouse model of multiple endocrine neoplasia type 2A (MEN2A). METHODS: Dynamic [(18)F]FDG and 6-[(18)F]F-L-DOPA small animal PET scans were acquired during 60 or 90 min in 8- to 20-month-old MEN2A transgenic mice. The kinetics of 6-[(18)F]F-L-DOPA, standardized uptake values (SUV) at 60 min and tumour volumes were recorded. The detection of MTCs using PET was confirmed by autopsy and histological analysis. RESULTS: 6-[(18)F]F-L-DOPA performs better than [(18)F]FDG for MTC detection in this transgenic mouse model. Uptake kinetics of 6-[(18)F]F-L-DOPA in MTCs are very different between mice but, in all cases, high contrast could be observed. Furthermore, 6-[(18)F]F-L-DOPA can detect tumours with sizes (1.8 mm(3)) that are near the resolution limit of PET, whereas they were undetectable by autopsy at the macroscopic level. CONCLUSION: 6-[(18)F]F-L-DOPA PET imaging can monitor the progression of MTCs in a genetically engineered mouse model.
PURPOSE:6-[(18)F]Fluoro-3,4-dihydroxy-L-phenylalanine (6-[(18)F]F-L-DOPA) is increasingly used for PET imaging of neuroendocrine tumours. In this study, we investigated the use of 6-[(18)F]F-L-DOPA to detect and to monitor the progression of medullary thyroid carcinoma (MTC) in a genetically engineered mouse model of multiple endocrine neoplasia type 2A (MEN2A). METHODS: Dynamic [(18)F]FDG and 6-[(18)F]F-L-DOPA small animal PET scans were acquired during 60 or 90 min in 8- to 20-month-old MEN2A transgenic mice. The kinetics of 6-[(18)F]F-L-DOPA, standardized uptake values (SUV) at 60 min and tumour volumes were recorded. The detection of MTCs using PET was confirmed by autopsy and histological analysis. RESULTS:6-[(18)F]F-L-DOPA performs better than [(18)F]FDG for MTC detection in this transgenicmouse model. Uptake kinetics of 6-[(18)F]F-L-DOPA in MTCs are very different between mice but, in all cases, high contrast could be observed. Furthermore, 6-[(18)F]F-L-DOPA can detect tumours with sizes (1.8 mm(3)) that are near the resolution limit of PET, whereas they were undetectable by autopsy at the macroscopic level. CONCLUSION:6-[(18)F]F-L-DOPA PET imaging can monitor the progression of MTCs in a genetically engineered mouse model.
Authors: Klaas P Koopmans; Elisabeth G E de Vries; Ido P Kema; Philip H Elsinga; Oliver C Neels; Wim J Sluiter; Anouk N A van der Horst-Schrivers; Pieter L Jager Journal: Lancet Oncol Date: 2006-09 Impact factor: 41.316
Authors: Henri J L M Timmers; Mohiuddin Hadi; Jorge A Carrasquillo; Clara C Chen; Lucia Martiniova; Millie Whatley; Alexander Ling; Graeme Eisenhofer; Karen T Adams; Karel Pacak Journal: J Nucl Med Date: 2007-09-14 Impact factor: 10.057
Authors: Oliver C Neels; Klaas P Koopmans; Pieter L Jager; Laya Vercauteren; Aren van Waarde; Janine Doorduin; Hetty Timmer-Bosscha; Adrienne H Brouwers; Elisabeth G E de Vries; Rudi A J O Dierckx; Ido P Kema; Philip H Elsinga Journal: Cancer Res Date: 2008-09-01 Impact factor: 12.701