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Literature DB >> 19652355

Expression, purification and preliminary X-ray crystallographic analysis of UDP-galactopyranose mutase from Deinococcus radiodurans.

Sarathy Karunan Partha¹, Sara A Bonderoff, Karin E van Straaten, David A R Sanders.

Abstract

UDP-galactopyranose mutase (UGM) catalyzes the interconversion of UDP-galactopyranose and UDP-galactofuranose. A UGM-substrate complex from Deinococccus radiodurans has been expressed, purified and crystallized. Crystals were obtained by the microbatch-under-oil method at room temperature. The crystals diffracted to 2.36 A resolution at the Canadian Light Source. The space group was found to be P2(1)2(1)2(1), with unit-cell parameters a = 134.0, b = 176.6, c = 221.6 A. The initial structure solution was determined by molecular replacement using UGM from Mycobacterium tuberculosis (PDB code 1v0j) as a template model.

Entities: Disease Species

Mesh：

Substances：

Year: 2009 PMID： 19652355 PMCID： PMC2720349 DOI： 10.1107/S1744309109027754

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

11 in total

Review 1. The bacterial cell wall as a source of antibacterial targets.

Authors: David W Green
Journal: Expert Opin Ther Targets Date: 2002-02 Impact factor: 6.902

2. Automated search-model discovery and preparation for structure solution by molecular replacement.

Authors: Ronan M Keegan; Martyn D Winn
Journal: Acta Crystallogr D Biol Crystallogr Date: 2007-03-16

Review 3. Galactofuranose metabolism: a potential target for antimicrobial chemotherapy.

Authors: L L Pedersen; S J Turco
Journal: Cell Mol Life Sci Date: 2003-02 Impact factor: 9.261

4. Use of TLS parameters to model anisotropic displacements in macromolecular refinement.

Authors: M D Winn; M N Isupov; G N Murshudov
Journal: Acta Crystallogr D Biol Crystallogr Date: 2001-01

5. Cell wall core galactofuran synthesis is essential for growth of mycobacteria.

Authors: F Pan; M Jackson; Y Ma; M McNeil
Journal: J Bacteriol Date: 2001-07 Impact factor: 3.490

6. UDP-galactopyranose mutase has a novel structure and mechanism.

Authors: D A Sanders; A G Staines; S A McMahon; M R McNeil; C Whitfield; J H Naismith
Journal: Nat Struct Biol Date: 2001-10

7. A new interpretation of the structure of the mycolyl-arabinogalactan complex of Mycobacterium tuberculosis as revealed through characterization of oligoglycosylalditol fragments by fast-atom bombardment mass spectrometry and 1H nuclear magnetic resonance spectroscopy.

Authors: G S Besra; K H Khoo; M R McNeil; A Dell; H R Morris; P J Brennan
Journal: Biochemistry Date: 1995-04-04 Impact factor: 3.162

8. Site-directed mutagenesis of UDP-galactopyranose mutase reveals a critical role for the active-site, conserved arginine residues.

Authors: Jennifer M Chad; Karunan Partha Sarathy; Todd D Gruber; Eshwari Addala; Laura L Kiessling; David A R Sanders
Journal: Biochemistry Date: 2007-05-19 Impact factor: 3.162

9. Crystal structures of Mycobacteria tuberculosis and Klebsiella pneumoniae UDP-galactopyranose mutase in the oxidised state and Klebsiella pneumoniae UDP-galactopyranose mutase in the (active) reduced state.

Authors: Konstantinos Beis; Velupillai Srikannathasan; Huanting Liu; Stephen W B Fullerton; Vicki A Bamford; David A R Sanders; Chris Whitfield; Mike R McNeil; James H Naismith
Journal: J Mol Biol Date: 2005-05-13 Impact factor: 5.469