| Literature DB >> 1965009 |
A I Glukhov1, S A Gordeev, S V Vinogradov, V I Kiselev, V M Kramarov, O I Kiselev, E S Severin.
Abstract
Using thermophilic DNA-polymerase from Thermus thermophilus we have amplified by polymerase chain reaction (PCR) specific DNA sequences of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV). DNA-polymerase from Thermus thermophilus (molecular mass of 80-86 kDa) differs in its physico-chemical properties from DNA-polymerase from Thermus aquaticus (molecular mass of 62-68 kDa). To amplify the specific EBV DNA sequence, oligonucleotide primers for the virus replicon region (oriP region) were used. As a result of amplification, a specific 405-bp DNA fragment was produced.Entities:
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Year: 1990 PMID： 1965009 DOI： 10.1016/0890-8508(90)90002-h
Source DB: PubMed Journal: Mol Cell Probes ISSN： 0890-8508 Impact factor: 2.365