Literature DB >> 19649724

Use of polyamidoamine dendrimers to engineer BDNF-producing human mesenchymal stem cells.

Antos Shakhbazau1, Dzmitry Shcharbin, Ihar Seviaryn, Natalya Goncharova, Svetlana Kosmacheva, Mihail Potapnev, Barbara Gabara, Maxim Ionov, Maria Bryszewska.   

Abstract

We report the use of polyamidoamine (PAMAM-NH(2)) dendrimers along with other non-viral vehicles for the in vitro transfection of human bone marrow mesenchymal stem cells (hMSCs) and for engineering MSCs to secrete brain-derived neurotrophic factor (BDNF). Different generations of cationic polyamidoamine dendrimers (generations 3-6) were tested on HEK 293T cells. hMSCs were then transfected with PAMAM-NH(2) G4 dendrimers and Lipofectamine 2000, which elicited the expression of GFP reporter in around 6 and 20% of the cells, respectively. Both vehicles were then shown to elicit the expression of BDNF in MSCs from a bicistronic cassette. Non-virally induced neurotrophin expression may be a safe and easy method for adapting autologous stem cells for therapeutic treatment of diseases and neural system injuries.

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Year:  2009        PMID： 19649724     DOI： 10.1007/s11033-009-9651-y

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  25 in total

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Authors:  Jose M Cosgaya; Jonah R Chan; Eric M Shooter
Journal:  Science       Date:  2002-11-08       Impact factor: 47.728

Review 2.  Dendrimers in biomedical applications--reflections on the field.

Authors:  Sönke Svenson; Donald A Tomalia
Journal:  Adv Drug Deliv Rev       Date:  2005-11-21       Impact factor: 15.470

3.  Axonal regeneration and functional recovery after complete spinal cord transection in rats by delayed treatment with transplants and neurotrophins.

Authors:  J V Coumans; T T Lin; H N Dai; L MacArthur; M McAtee; C Nash; B S Bregman
Journal:  J Neurosci       Date:  2001-12-01       Impact factor: 6.167

Review 4.  Neurotrophin-regulated signalling pathways.

Authors:  Louis F Reichardt
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2006-09-29       Impact factor: 6.237

Review 5.  Molecular targets to promote central nervous system regeneration.

Authors:  Gino B Ferraro; Yazan Z Alabed; Alyson E Fournier
Journal:  Curr Neurovasc Res       Date:  2004-01       Impact factor: 1.990

6.  Functional recovery in traumatic spinal cord injury after transplantation of multineurotrophin-expressing glial-restricted precursor cells.

Authors:  Qilin Cao; Xiao-Ming Xu; William H Devries; Gaby U Enzmann; Peipei Ping; Pantelis Tsoulfas; Patrick M Wood; Mary Bartlett Bunge; Scott R Whittemore
Journal:  J Neurosci       Date:  2005-07-27       Impact factor: 6.167

7.  Treatment of the chronically injured spinal cord with neurotrophic factors can promote axonal regeneration from supraspinal neurons.

Authors:  J H Ye; J D Houle
Journal:  Exp Neurol       Date:  1997-01       Impact factor: 5.330

8.  Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectors.

Authors:  José Luís Santos; Elena Oramas; Ana Paula Pêgo; Pedro Lopes Granja; Helena Tomás
Journal:  J Control Release       Date:  2008-11-24       Impact factor: 9.776

9.  Neuroprotective effects of glial cell line-derived neurotrophic factor mediated by an adeno-associated virus vector in a transgenic animal model of amyotrophic lateral sclerosis.

Authors:  Li-Jun Wang; Yan-Yan Lu; Shin-ichi Muramatsu; Kunihiko Ikeguchi; Ken-ichi Fujimoto; Takashi Okada; Hiroaki Mizukami; Takashi Matsushita; Yutaka Hanazono; Akihiro Kume; Toshiharu Nagatsu; Keiya Ozawa; Imaharu Nakano
Journal:  J Neurosci       Date:  2002-08-15       Impact factor: 6.167

10.  Muscle-specific microRNA miR-206 promotes muscle differentiation.

Authors:  Hak Kyun Kim; Yong Sun Lee; Umasundari Sivaprasad; Ankit Malhotra; Anindya Dutta
Journal:  J Cell Biol       Date:  2006-08-21       Impact factor: 10.539
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  10 in total

1.  Microarray analysis of cultured rat hippocampal neurons treated with brain derived neurotrophic factor.

Authors:  Chiara Cazzin; Silvia Mion; Fabrizio Caldara; Joseph M Rimland; Enrico Domenici
Journal:  Mol Biol Rep       Date:  2010-06-10       Impact factor: 2.316

2.  Recombinant expression of human nerve growth factor beta in rabbit bone marrow mesenchymal stem cells.

Authors:  Bo-Sheng Fan; Ji-Yu Lou
Journal:  Mol Biol Rep       Date:  2010-03-17       Impact factor: 2.316

3.  CD133+ CD44+ subgroups may be human small intestinal stem cells.

Authors:  Neng-Yi Hou; Kun Yang; Tie Chen; Xin-Zu Chen; Bo Zhang; Xian-Ming Mo; Jian-Kun Hu
Journal:  Mol Biol Rep       Date:  2010-06-06       Impact factor: 2.316

4.  The association of CLOCK gene T3111C polymorphism and hPER3 gene 54-nucleotide repeat polymorphism with Chinese Han people schizophrenics.

Authors:  Jing Zhang; Ga Liao; Chang Liu; Lei Sun; Yanyou Liu; Yuhui Wang; Zhou Jiang; Zhengrong Wang
Journal:  Mol Biol Rep       Date:  2010-04-03       Impact factor: 2.316

5.  Expression of Pdx1 mediates differentiation from mesenchymal stem cells into insulin-producing cells.

Authors:  Huijuan Yuan; Jie Li; Ning Xin; Zhigang Zhao; Guijun Qin
Journal:  Mol Biol Rep       Date:  2010-03-21       Impact factor: 2.316

6.  The restoration of full-thickness cartilage defects with mesenchymal stem cells (MSCs) loaded and cross-linked bilayer collagen scaffolds on rabbit model.

Authors:  Yiying Qi; Tengfei Zhao; Ke Xu; Tianyang Dai; Weiqi Yan
Journal:  Mol Biol Rep       Date:  2011-05-19       Impact factor: 2.316

7.  Efficient preparation and labeling of human induced pluripotent stem cells by nanotechnology.

Authors:  Jing Ruan; Jie Shen; Zheng Wang; Jiajia Ji; Hua Song; Kan Wang; Bin Liu; Jinhui Li; Daxiang Cui
Journal:  Int J Nanomedicine       Date:  2011-02-21

8.  Fourth generation phosphorus-containing dendrimers: prospective drug and gene delivery carrier.

Authors:  D Shcharbin; V Dzmitruk; A Shakhbazau; N Goncharova; I Seviaryn; S Kosmacheva; M Potapnev; E Pedziwiatr-Werbicka; M Bryszewska; M Talabaev; A Chernov; V Kulchitsky; A-M Caminade; J-P Majoral
Journal:  Pharmaceutics       Date:  2011-08-05       Impact factor: 6.321

Review 9.  Use of Polyamidoamine Dendrimers in Brain Diseases.

Authors:  Maria Florendo; Alexander Figacz; Bhairavi Srinageshwar; Ajit Sharma; Douglas Swanson; Gary L Dunbar; Julien Rossignol
Journal:  Molecules       Date:  2018-09-03       Impact factor: 4.411

10.  Generation Dependent Effects and Entrance to Mitochondria of Hybrid Dendrimers on Normal and Cancer Neuronal Cells In Vitro.

Authors:  Aleksandra Szwed; Katarzyna Miłowska; Sylwia Michlewska; Silvia Moreno; Dzmitry Shcharbin; Rafael Gomez-Ramirez; Francisco Javier de la Mata; Jean-Pierre Majoral; Maria Bryszewska; Teresa Gabryelak
Journal:  Biomolecules       Date:  2020-03-09
  10 in total

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