Gordonia hankookensis sp. nov., isolated from soil.

A Gram-positive, aerobic and non-motile bacterial strain, designated ON-33(T), was subjected to a study based on a polyphasic approach to determine its exact taxonomic position. Strain ON-33(T) grew optimally at pH 7.0-7.5 and 30 degrees C. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain ON-33(T) fell within the clade comprising Gordonia species, clustering with Gordonia soli CC-AB07(T), with which it exhibited 16S rRNA gene sequence similarity of 98.5 %. The chemotaxonomic properties of strain ON-33(T) were consistent with those shared by members of the genus Gordonia. The peptidoglycan type was based on meso-diaminopimelic acid and the whole-cell sugars were arabinose and galactose. The predominant menaquinone was MK-9(H(2)). The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were C(16 : 0), iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c, 10-methyl C(18 : 0) and C(18 : 1)omega9c. The DNA G+C content was 66.9 mol%. Strain ON-33(T) exhibited a DNA-DNA relatedness value of 13 % to G. soli DSM 44995(T) and could be differentiated from G. soli by differences in phenotypic characteristics. On the basis of the data obtained, strain ON-33(T) is considered to represent a novel species of the genus Gordonia, for which the name Gordonia hankookensis sp. nov., is proposed. The type strain is ON-33(T) (=KCTC 19599(T)=CCUG 57507(T)).