Kineosporia mesophila sp. nov., isolated from surface-sterilized stems of Tripterygium wilfordii.

An endophytic actinomycete strain, designated YIM 65293(T), was isolated from a surface-sterilized stem sample of Tripterygium wilfordii collected from Yunnan province, south-west China, and its taxonomic position was investigated. The chemical and morphological properties of the organism were consistent with those of the genus Kineosporia. Phylogenetic analysis indicated that the levels of 16S rRNA gene sequence similarity between strain YIM 65293(T) and other type strains of recognized members of the genus Kineosporia were 97.0-98.2 %. However, the DNA-DNA hybridization values, in combination with differences in phenotypic characteristics, revealed that the strain differed from recognized species of the genus Kineosporia. Therefore, strain YIM 65293(T) represents a novel species of the genus Kineosporia, for which the name Kineosporia mesophila sp. nov. is proposed. The type strain is YIM 65293(T) (=CCTCC AA 208061(T)=DSM 45271(T)).