| Literature DB >> 19643144 |
Jiangtao Xu1, Zhenmei Zhang, Yanbo Yin, Shangjin Cui, Shouzhen Xu, Yanyan Guo, Jida Li, Jianlin Wang, Xingcai Liu, Limin Han.
Abstract
To establish a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of infectious bursal disease virus (IBDV), four primers specific to six regions of the VP3 gene were designed; the VP3 region was selected because it is a conserved part of the IBDV genome. After amplification in an isothermal water bath for 70 min, samples containing IBDV generated the expected ladder-like products while other viruses generated no product. The sensitivity and specificity of the RT-LAMP assay were evaluated by comparison with reverse-transcription polymerase chain reaction (RT-PCR) and virus isolation. The assay was significantly more sensitive than normal gel-based RT-PCR. Because it is specific and simple, the RT-LAMP assay can be widely applied in clinical laboratories for rapid detection of IBVD.Entities:
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Year: 2009 PMID： 19643144 DOI： 10.1016/j.jviromet.2009.07.010
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014