Literature DB >> 19642864

Ex vivo serotype-specific transduction of equine joint tissue by self-complementary adeno-associated viral vectors.

L R Goodrich1, V W Choi, B A Duda Carbone, C W McIlwraith, R J Samulski.   

Abstract

Cell transplantation for the treatment of joint disease is an important clinical tool. Genetic modification of cells before transplantation has shown enhanced healing. Ex vivo genetic modification of joint tissue cells with various adeno-associated virus (AAV) serotypes has not been investigated. The transduction efficiencies of self-complementary AAV serotypes (1-6 and 8) were determined in joint tissue containing chondrocytes and synoviocytes isolated from equine models. When comparing scAAV serotypes for efficient transduction ex vivo, in chondrocytes versus synoviocytes, serotypes 6 and 2, and serotypes 3 and 2, respectively, appeared superior for gene expression. Unlike adenoviral vectors, no upregulation of inflammatory markers, such as matrix metalloproteinases and aggrecanase, was seen on treatment of joint tissue with AAV vectors ex vivo. Our findings also corroborate that ex vivo transduction of joint tissue can result in high transgene protein levels over time, and transplantation modalities might be feasible using AAV vectors in the treatment of joint-related diseases.

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Year:  2009        PMID： 19642864      PMCID： PMC2861962          DOI： 10.1089/hum.2009.030

Source DB:  PubMed          Journal:  Hum Gene Ther        ISSN： 1043-0342            Impact factor:   5.695


  37 in total

Review 1.  New recombinant serotypes of AAV vectors.

Authors:  Guangping Gao; Luk H Vandenberghe; James M Wilson
Journal:  Curr Gene Ther       Date:  2005-06       Impact factor: 4.391

2.  Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis.

Authors:  D M McCarty; P E Monahan; R J Samulski
Journal:  Gene Ther       Date:  2001-08       Impact factor: 5.250

3.  In vivo gene delivery to synovium by lentiviral vectors.

Authors:  Elvire Gouze; Robert Pawliuk; Carmencita Pilapil; Jean-Noel Gouze; Christina Fleet; Glyn D Palmer; Christopher H Evans; Philippe Leboulch; Steven C Ghivizzani
Journal:  Mol Ther       Date:  2002-04       Impact factor: 11.454

4.  Direct adenovirus-mediated gene transfer of interleukin 1 and tumor necrosis factor alpha soluble receptors to rabbit knees with experimental arthritis has local and distal anti-arthritic effects.

Authors:  S C Ghivizzani; E R Lechman; R Kang; C Tio; J Kolls; C H Evans; P D Robbins
Journal:  Proc Natl Acad Sci U S A       Date:  1998-04-14       Impact factor: 11.205

5.  Direct adenovirus-mediated insulin-like growth factor I gene transfer enhances transplant chondrocyte function.

Authors:  B D Brower-Toland; R A Saxer; L R Goodrich; Z Mi; P D Robbins; C H Evans; A J Nixon
Journal:  Hum Gene Ther       Date:  2001-01-20       Impact factor: 5.695

6.  Adenovirus-mediated gene transfer of glutamine: fructose-6-phosphate amidotransferase antagonizes the effects of interleukin-1beta on rat chondrocytes.

Authors:  J-N Gouze; E Gouze; G D Palmer; H Kaneto; S C Ghivizzani; A J Grodzinsky; C H Evans
Journal:  Osteoarthritis Cartilage       Date:  2004-03       Impact factor: 6.576

Review 7.  Rate limiting steps of AAV transduction and implications for human gene therapy.

Authors:  S Sanlioglu; M M Monick; G Luleci; G W Hunninghake; J F Engelhardt
Journal:  Curr Gene Ther       Date:  2001-07       Impact factor: 4.391

Review 8.  Gene therapies for osteoarthritis.

Authors:  Christopher H Evans
Journal:  Curr Rheumatol Rep       Date:  2004-02       Impact factor: 4.592

9.  Recombinant adeno-associated virus vectors efficiently and persistently transduce chondrocytes in normal and osteoarthritic human articular cartilage.

Authors:  Henning Madry; Magali Cucchiarini; Ernest F Terwilliger; Stephen B Trippel
Journal:  Hum Gene Ther       Date:  2003-03-01       Impact factor: 5.695

10.  Genetic modification of chondrocytes with insulin-like growth factor-1 enhances cartilage healing in an equine model.

Authors:  L R Goodrich; C Hidaka; P D Robbins; C H Evans; A J Nixon
Journal:  J Bone Joint Surg Br       Date:  2007-05
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  21 in total

Review 1.  Self-complementary adeno-associated viral vectors for gene therapy of hemophilia B: progress and challenges.

Authors:  Deepak Raj; Andrew M Davidoff; Amit C Nathwani
Journal:  Expert Rev Hematol       Date:  2011-10       Impact factor: 2.929

2.  scAAVIL-1ra dosing trial in a large animal model and validation of long-term expression with repeat administration for osteoarthritis therapy.

Authors:  L R Goodrich; J C Grieger; J N Phillips; N Khan; S J Gray; C W McIlwraith; R J Samulski
Journal:  Gene Ther       Date:  2015-04-23       Impact factor: 5.250

3.  Single intra-articular injection of adeno-associated virus results in stable and controllable in vivo transgene expression in normal rat knees.

Authors:  K A Payne; H H Lee; A M Haleem; C Martins; Z Yuan; C Qiao; X Xiao; C R Chu
Journal:  Osteoarthritis Cartilage       Date:  2011-04-28       Impact factor: 6.576

Review 4.  Use of tissue engineering strategies to repair joint tissues in osteoarthritis: viral gene transfer approaches.

Authors:  Magali Cucchiarini; Henning Madry
Journal:  Curr Rheumatol Rep       Date:  2014-10       Impact factor: 4.592

5.  Humoral and cell-mediated immune response, and growth factor synthesis after direct intraarticular injection of rAAV2-IGF-I and rAAV5-IGF-I in the equine middle carpal joint.

Authors:  Kyla Ortved; Bettina Wagner; Roberto Calcedo; James Wilson; Deanna Schaefer; Alan Nixon
Journal:  Hum Gene Ther       Date:  2015-03       Impact factor: 5.695

Review 6.  Gene Delivery to Joints by Intra-Articular Injection.

Authors:  Christopher H Evans; Steven C Ghivizzani; Paul D Robbins
Journal:  Hum Gene Ther       Date:  2018-01       Impact factor: 5.695

Review 7.  Arthritis gene therapy and its tortuous path into the clinic.

Authors:  Christopher H Evans; Steven C Ghivizzani; Paul D Robbins
Journal:  Transl Res       Date:  2013-01-29       Impact factor: 7.012

8.  scAAV-mediated gene transfer of interleukin-1-receptor antagonist to synovium and articular cartilage in large mammalian joints.

Authors:  R S Watson; T A Broome; P P Levings; B L Rice; J D Kay; A D Smith; E Gouze; J-N Gouze; E A Dacanay; W W Hauswirth; D M Nickerson; M J Dark; P T Colahan; S C Ghivizzani
Journal:  Gene Ther       Date:  2012-11-15       Impact factor: 5.250

9.  The horse as a model of naturally occurring osteoarthritis.

Authors:  C W McIlwraith; D D Frisbie; C E Kawcak
Journal:  Bone Joint Res       Date:  2012-11-01       Impact factor: 5.853

10.  Optimization of scAAVIL-1ra In Vitro and In Vivo to Deliver High Levels of Therapeutic Protein for Treatment of Osteoarthritis.

Authors:  Laurie R Goodrich; Jennifer N Phillips; C Wayne McIlwraith; Stacey B Foti; Joshua C Grieger; Steven J Gray; R Jude Samulski
Journal:  Mol Ther Nucleic Acids       Date:  2013-02-05       Impact factor: 10.183
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