| Literature DB >> 1963950 |
Abstract
In order to select insertion sequences able to promote transcription of flanking genes (ISp elements), three mobilizable RSF1010 derived vectors were constructed. Using promoterless antibiotic resistance genes, ISp elements ranging from 0.75 to 2.9 kb were isolated from Escherichia coli and Rhizobium meliloti. Restriction and hybridization experiments revealed that identical ISp elements could be isolated from different R. meliloti strains and that one of these is similar to an insertion sequence found previously in R. meliloti 2011.Entities:
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Year: 1990 PMID: 1963950 DOI: 10.1016/0147-619x(90)90007-y
Source DB: PubMed Journal: Plasmid ISSN: 0147-619X Impact factor: 3.466