Literature DB >> 19637239

Accurate quantitation of standard peptides used for quantitative proteomics.

Narisa K Bordeerat1, Nadia I Georgieva, David G Klapper, Leonard B Collins, Tyra J Cross, Christoph H Borchers, James A Swenberg, Gunnar Boysen.   

Abstract

MS-based proteomics has become an indispensable tool in system biology generating a need for accurate and precise quantitation of peptide standards. The presented method utilizes ultra performance LC-MS/MS (UPLC-MS/MS) to accurately quantify peptide standards at concentrations of 0.1-10 microM. The ability for accurate quantitation of micro-molar concentrations has the advantages that quantitation can be performed routinely with high precision and the high sensitivity of the method minimizes the amounts required.

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Year:  2009        PMID： 19637239      PMCID： PMC2766299          DOI： 10.1002/pmic.200900043

Source DB:  PubMed          Journal:  Proteomics        ISSN： 1615-9853            Impact factor:   3.984


  24 in total

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2.  Formation of 1,2:3,4-diepoxybutane-specific hemoglobin adducts in 1,3-butadiene exposed workers.

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6.  In silico proteome-wide amino aCid and elemental composition (PACE) analysis of expression proteomics data provides a fingerprint of dominant metabolic processes.

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  6 in total

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