AIMS OF THE STUDY: Wounds represent a major health burden and drain on healthcare resources in the world including Ghana and Africa. The majority of the people of Ghana and Africa still patronize traditional medicine for their health needs including various forms of wounds. The aim of this study is the identification of medicinal plants, type of wounds, dosage forms and collection methods used traditionally in treating wounds in the Bosomtwi-Atwima-Kwanwoma district, Ghana. In vitro screening of selected extracts from these plants on cell physiology of human dermal fibroblasts and keratinocytes was to be performed. MATERIALS AND METHODS: Validated questionnaires were administered to 78 traditional healers in 54 communities of the district. Interviews and structured conversations were used to administer the questionnaires. Selected herbal material dominantly used by the healers was collected, identified and aqueous and ethanolic extracts were investigated in vitro on influence on cell physiology of keratinocytes and dermal fibroblasts (MTT-, BrdU-, LDH-assay). Antioxidant activities of ethanolic extracts were determined by free radical scavenging activity. Antiadhesive activity against Helicobacter pylori on human stomach cells was investigated for extracts reported to be used for stomach ulcer treatment. RESULTS: The ethnopharmacological survey revealed 104 plants species belonging to 47 families. The detailed use of these plants is documented. Aqueous extracts of Phyllanthus muellerianus, Pycnanthus angolensis and Combretum smeathmanni influenced the mitochondrial activity and proliferation of dermal fibroblasts and keratinocytes significantly. Ethanolic extracts of selected plants exhibited strong antioxidant activities comparable to alpha-tocopherol. For Spathodea campanulata, Hoslundia opposita and Pycnanthus angolensis, which were reported by the healers to be used also for wound healing in case of stomach ulcers, strong antiadhesive activity against Helicobacter pylori was demonstrated, while the extracts did not exhibit any direct cytotoxicity against the bacterium. CONCLUSIONS: Traditional use of many wound-healing plants from Ghana can be well rationalized by the in vitro investigation of aqueous extracts. E.g. extracts of Phyllanthus muellerianus, Pycnanthus angolensis and Combretum smeathmanni exhibited significant influence on the cell viability and proliferation of keratinocytes and dermal fibroblasts.
AIMS OF THE STUDY: Wounds represent a major health burden and drain on healthcare resources in the world including Ghana and Africa. The majority of the people of Ghana and Africa still patronize traditional medicine for their health needs including various forms of wounds. The aim of this study is the identification of medicinal plants, type of wounds, dosage forms and collection methods used traditionally in treating wounds in the Bosomtwi-Atwima-Kwanwoma district, Ghana. In vitro screening of selected extracts from these plants on cell physiology of human dermal fibroblasts and keratinocytes was to be performed. MATERIALS AND METHODS: Validated questionnaires were administered to 78 traditional healers in 54 communities of the district. Interviews and structured conversations were used to administer the questionnaires. Selected herbal material dominantly used by the healers was collected, identified and aqueous and ethanolic extracts were investigated in vitro on influence on cell physiology of keratinocytes and dermal fibroblasts (MTT-, BrdU-, LDH-assay). Antioxidant activities of ethanolic extracts were determined by free radical scavenging activity. Antiadhesive activity against Helicobacter pylori on human stomach cells was investigated for extracts reported to be used for stomach ulcer treatment. RESULTS: The ethnopharmacological survey revealed 104 plants species belonging to 47 families. The detailed use of these plants is documented. Aqueous extracts of Phyllanthus muellerianus, Pycnanthus angolensis and Combretum smeathmanni influenced the mitochondrial activity and proliferation of dermal fibroblasts and keratinocytes significantly. Ethanolic extracts of selected plants exhibited strong antioxidant activities comparable to alpha-tocopherol. For Spathodea campanulata, Hoslundia opposita and Pycnanthus angolensis, which were reported by the healers to be used also for wound healing in case of stomach ulcers, strong antiadhesive activity against Helicobacter pylori was demonstrated, while the extracts did not exhibit any direct cytotoxicity against the bacterium. CONCLUSIONS: Traditional use of many wound-healing plants from Ghana can be well rationalized by the in vitro investigation of aqueous extracts. E.g. extracts of Phyllanthus muellerianus, Pycnanthus angolensis and Combretum smeathmanni exhibited significant influence on the cell viability and proliferation of keratinocytes and dermal fibroblasts.
Authors: C N Fokunang; V Ndikum; O Y Tabi; R B Jiofack; B Ngameni; N M Guedje; E A Tembe-Fokunang; P Tomkins; S Barkwan; F Kechia; E Asongalem; J Ngoupayou; N J Torimiro; K H Gonsu; V Sielinou; B T Ngadjui; F Angwafor; A Nkongmeneck; O M Abena; J Ngogang; T Asonganyi; V Colizzi; J Lohoue Journal: Afr J Tradit Complement Altern Med Date: 2011-04-02
Authors: Dieudonné Ndjonka; Bärbel Bergmann; Christian Agyare; Flávia M Zimbres; Kai Lüersen; Andreas Hensel; Carsten Wrenger; Eva Liebau Journal: Parasitol Res Date: 2012-04-05 Impact factor: 2.289
Authors: Gustav Komlaga; Sandrine Cojean; Rita A Dickson; Mehdi A Beniddir; Soulaf Suyyagh-Albouz; Merlin L K Mensah; Christian Agyare; Pierre Champy; Philippe M Loiseau Journal: Parasitol Res Date: 2016-05-13 Impact factor: 2.289
Authors: Kieran S O'Brien; Amr S Soliman; Kofi Annan; Richard N Lartey; Baffour Awuah; Sofia D Merajver Journal: J Cancer Educ Date: 2012-06 Impact factor: 2.037
Authors: K Amegbor; K Metowogo; K Eklu-Gadegbeku; A Agbonon; K A Aklikokou; G Napo-Koura; M Gbeassor Journal: Afr J Tradit Complement Altern Med Date: 2012-07-01