Literature DB >> 19635503

A multiplex PCR protocol for rapid identification of Candida glabrata and its phylogenetically related species Candida nivariensis and Candida bracarensis.

Orazio Romeo1, Fabio Scordino, Ida Pernice, Carla Lo Passo, Giuseppe Criseo.   

Abstract

We have developed a multiplex PCR protocol for the detection of Candida glabrata and its closely related species Candida nivariensis and Candida bracarensis. The method uses four PCR primers, targeting the ITS1 region and the 5.8S ribosomal RNA gene. The combination of these primers yielded unique results to all Candida species tested. The PCR assay we developed was found to be a rapid, specific and easy to perform method and it will be useful for characterizing large numbers of isolates for epidemiological studies.

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Year:  2009        PMID： 19635503     DOI： 10.1016/j.mimet.2009.07.016

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  20 in total

1.  Occurrence and characterization of Candida nivariensis from a culture collection of Candida glabrata clinical isolates in Malaysia.

Authors:  Sun Tee Tay; Azadeh Lotfalikhani; Negar Shafiei Sabet; Sasheela Ponnampalavanar; Sofiah Sulaiman; Shiang Ling Na; Kee Peng Ng
Journal:  Mycopathologia       Date:  2014-07-15       Impact factor: 2.574

2.  Rapid discrimination between Candida glabrata, Candida nivariensis, and Candida bracarensis by use of a singleplex PCR.

Authors:  A Enache-Angoulvant; J Guitard; F Grenouillet; T Martin; P Durrens; C Fairhead; C Hennequin
Journal:  J Clin Microbiol       Date:  2011-07-13       Impact factor: 5.948

3.  Development of two molecular approaches for differentiation of clinically relevant yeast species closely related to Candida guilliermondii and Candida famata.

Authors:  Xiaobo Feng; Jingsong Wu; Bo Ling; Xianwei Yang; Wanqing Liao; Weihua Pan; Zhirong Yao
Journal:  J Clin Microbiol       Date:  2014-06-20       Impact factor: 5.948

4.  Prevalence of Candida nivariensis and Candida bracarensis in vulvovaginal Candidiasis.

Authors:  Jianling Li; Yingying Shan; Shangrong Fan; Xiaoping Liu
Journal:  Mycopathologia       Date:  2014-08-15       Impact factor: 2.574

5.  Differentiation of Candida glabrata, C. nivariensis and C. bracarensis based on fragment length polymorphism of ITS1 and ITS2 and restriction fragment length polymorphism of ITS and D1/D2 regions in rDNA.

Authors:  H Mirhendi; B Bruun; H C Schønheyder; J J Christensen; K Fuursted; B Gahrn-Hansen; H K Johansen; L Nielsen; J D Knudsen; M C Arendrup
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-05-24       Impact factor: 3.267

6.  Candida palmioleophila: characterization of a previously overlooked pathogen and its unique susceptibility profile in comparison with five related species.

Authors:  Rasmus H Jensen; Maiken C Arendrup
Journal:  J Clin Microbiol       Date:  2010-12-08       Impact factor: 5.948

7.  Species distribution and in vitro antifungal susceptibility profiles of yeast isolates from invasive infections during a Portuguese multicenter survey.

Authors:  I Faria-Ramos; J Neves-Maia; E Ricardo; J Santos-Antunes; A T Silva; S Costa-de-Oliveira; E Cantón; A G Rodrigues; C Pina-Vaz
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2014-07-11       Impact factor: 3.267

8.  Multilocus microsatellite analysis of European and African Candida glabrata isolates.

Authors:  V Chillemi; C Lo Passo; A D van Diepeningen; S Rharmitt; D Delfino; A Cascio; N E Nnadi; B D Cilo; P Sampaio; H-J Tietz; J Pemán; G Criseo; O Romeo; F Scordino
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2016-03-05       Impact factor: 3.267

9.  Candida glabrata complex from patients with healthcare-associated infections in Mansoura University Hospitals, Egypt: distribution, antifungal susceptibility and effect of fluconazole and polymyxin B combination.

Authors:  Ghada Mashaly; Raghdaa Shrief
Journal:  Germs       Date:  2019-09-02

10.  Analysis of Biofilm-Related Genes and Antifungal Susceptibility Pattern of Vaginal Candida albicans and Non-Candida albicans Species.

Authors:  Faezeh Mohammadi; Nima Hemmat; Zahra Bajalan; Amir Javadi
Journal:  Biomed Res Int       Date:  2021-05-28       Impact factor: 3.411
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