Bi-Hua Bie1, Yi-Hong Zhang, Zhi-Qi Zhao. 1. Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China.
Abstract
OBJECTIVE: The aim of the present study is to verify the ATP-induced varied responses in isolated dorsal root ganglion (DRG) neurons of the adult rat, and investigate the modulatory effects of specific P2X receptor agonist beta, gamma-me-ATP and protein kinase C (PKC) on P2X receptor-mediated inward current in DRG neurons. METHODS: Whole cell patch-clamp was employed to record the currents on acutely isolated DRG neurons in the adult rats. RESULTS: beta, gamma-me-ATP, similar as ATP, evoked 2 distinct subtypes of P2X receptor-mediated inward currents in a dose-dependent manner in DRG neurons. Activation of PKC by phorbol 12, 13-dibutyrate (PDBu) significantly inhibited both subtypes of inward currents mediated by P2X receptors in a dose-dependent manner. CONCLUSION: Activation of PKC negatively modulated the P2X receptor-mediated currents in rat DRG neurons, which may be of benefit to preventing the over-excitation of nociceptor under inflammatory or neuropathic conditions.
OBJECTIVE: The aim of the present study is to verify the ATP-induced varied responses in isolated dorsal root ganglion (DRG) neurons of the adult rat, and investigate the modulatory effects of specific P2X receptor agonist beta, gamma-me-ATP and protein kinase C (PKC) on P2X receptor-mediated inward current in DRG neurons. METHODS: Whole cell patch-clamp was employed to record the currents on acutely isolated DRG neurons in the adult rats. RESULTS:beta, gamma-me-ATP, similar as ATP, evoked 2 distinct subtypes of P2X receptor-mediated inward currents in a dose-dependent manner in DRG neurons. Activation of PKC by phorbol 12, 13-dibutyrate (PDBu) significantly inhibited both subtypes of inward currents mediated by P2X receptors in a dose-dependent manner. CONCLUSION: Activation of PKC negatively modulated the P2X receptor-mediated currents in rat DRG neurons, which may be of benefit to preventing the over-excitation of nociceptor under inflammatory or neuropathic conditions.
Authors: D A Cockayne; S G Hamilton; Q M Zhu; P M Dunn; Y Zhong; S Novakovic; A B Malmberg; G Cain; A Berson; L Kassotakis; L Hedley; W G Lachnit; G Burnstock; S B McMahon; A P Ford Journal: Nature Date: 2000-10-26 Impact factor: 49.962