| Literature DB >> 19629726 |
Masahiko Inoue1, Yoshihiro Wakayama, Hiroko Kojima, Seiji Shibuya, Takahiro Jimi, Hajime Hara, Shoji Iijima, Hisatsugu Masaki, Hiroaki Oniki, Yoko Matsuzaki.
Abstract
The examination was performed whether aquaporin (AQP) 9 is expressed in normal skeletal muscle at mRNA and protein levels. Gel electrophoresis of the reverse transcription-polymerase chain reaction (RT-PCR) product of total RNA samples of human normal muscles by oligonucleotide primers for human AQP9 showed a band of 221 basepairs, which corresponded to the basepair length between two primers of AQP9. The nucleotide sequence of RT-PCR product coincided with that of human AQP9. Immunoblot analysis revealed that the rabbit and sheep antibodies against the synthetic peptide of the C-terminal cytoplasmic domain of human AQP9 molecule reacted with a protein of approximately 30 kDa molecular weight in extracts of human normal skeletal muscles. Immunohistochemistry with our anti-AQP9 antibodies showed an immunoreaction at the myofiber surface of both type 1 and type 2 fibers with almost equal staining intensity in human skeletal muscles. The implication of AQP9 expression in skeletal myofibers was discussed.Entities:
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Year: 2009 PMID： 19629726 DOI： 10.1007/s10735-009-9226-1
Source DB: PubMed Journal: J Mol Histol ISSN： 1567-2379 Impact factor: 2.611