| Literature DB >> 19628007 |
Valery Grinevich1, Alexander Kolleker, Marina Eliava, Naoki Takada, Hiroshi Takuma, Yugo Fukazawa, Ryuichi Shigemoto, Dietmar Kuhl, Jack Waters, Peter H Seeburg, Pavel Osten.
Abstract
The brain-specific immediate early gene Arc/Arg3.1 is induced in response to a variety of stimuli, including sensory and behavior-linked neural activity. Here we report the generation of transgenic mice, termed TgArc/Arg3.1-d4EGFP, expressing a 4-h half-life form of enhanced green fluorescent protein (d4EGFP) under the control of the Arc/Arg3.1 promoter. We show that d4EGFP-mediated fluorescence faithfully reports Arc/Arg3.1 induction in response to physiological, pathological and pharmacological stimuli, and that this fluorescence permits electrical recording from activated neurons in the live mouse. Moreover, the fluorescent Arc/Arg3.1 indicator revealed activity changes in circumscribed brain areas in distinct modes of stress and in a mouse model of Alzheimer's disease. These findings identify the TgArc/Arg3.1-d4EGFP mouse as a versatile tool to monitor Arc/Arg3.1 induction in neural circuits, both in vitro and in vivo.Entities:
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Year: 2009 PMID： 19628007 PMCID： PMC4022775 DOI： 10.1016/j.jneumeth.2009.07.015
Source DB: PubMed Journal: J Neurosci Methods ISSN： 0165-0270 Impact factor: 2.390