Literature DB >> 19627979

Application of the Van Slyke-Cullen irreversible mechanism in the analysis of enzymatic progress curves.

Petr Kuzmic1.   

Abstract

For enzymatic progress curves conforming to the Michaelis-Menten mechanism E+Sright harpoon over left harpoonES-->E+P, the minimal fitting model cast as a system of numerically integrated differential equations is the simplified, irreversible Van Slyke-Cullen mechanism E+S-->ES-->E+P. The best-fit value of the bimolecular association rate constant is identical to the specificity constant kcat/KM. An illustrative example involves a fluorogenic continuous assay of the HIV protease, analyzed by the differential-equation oriented software package DYNAFIT [P. Kuzmic, Anal. Biochem. 237 (1996) 260].

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Year:  2009        PMID： 19627979     DOI： 10.1016/j.ab.2009.06.040

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  12 in total

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4.  Full length and protease domain activity of chikungunya virus nsP2 differ from other alphavirus nsP2 proteases in recognition of small peptide substrates.

Authors:  Chonticha Saisawang; Pornpan Sillapee; Kwanhathai Sinsirimongkol; Sukathida Ubol; Duncan R Smith; Albert J Ketterman
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5.  Serum Albumin Binding and Esterase Activity: Mechanistic Interactions with Organophosphates.

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6.  Rapid Mechanistic Evaluation and Parameter Estimation of Putative Inhibitors in a Single-Step Progress-Curve Analysis: The Case of Horse Butyrylcholinesterase.

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Journal:  Molecules       Date:  2017-07-26       Impact factor: 4.411

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Review 9.  Excision of Oxidatively Generated Guanine Lesions by Competitive DNA Repair Pathways.

Authors:  Vladimir Shafirovich; Nicholas E Geacintov
Journal:  Int J Mol Sci       Date:  2021-03-07       Impact factor: 5.923

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Journal:  Comput Struct Biotechnol J       Date:  2021-04-17       Impact factor: 7.271
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