| Literature DB >> 19619511 |
Bernd Sokolowski1, R Keith Duncan, Stephanie Chen, Jörg Karolat, Thandavarayan Kathiresan, Margaret Harvey.
Abstract
Owing to the multifaceted functions of the large conductance Ca(2+)-activated K(+) channel (BK), identification of protein-protein interactions is essential in determining BK regulation. A yeast two-hybrid screening of a cochlear cDNA library revealed a BK-ApoA1 interaction. Patch clamp recordings of excised membrane patches from transfected HEK293 cells showed that ApoA1 inhibits the BK alpha-subunit by significantly increasing activation and deactivation times, and shifting half-activation voltage to more positive potentials. Reciprocal coimmunoprecipitations verified the BK-ApoA1 interaction using excised sensory epithelium and ganglia. Additionally, immunocolocalization studies revealed BK and ApoA1 expression in both receptor cells and auditory neurons. These data suggest new avenues of investigation, given the importance of apolipoproteins in neurological diseases.Entities:
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Year: 2009 PMID： 19619511 PMCID： PMC2757790 DOI： 10.1016/j.bbrc.2009.07.074
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575