| Literature DB >> 19605586 |
Salomé González-Reyes1, Alberto García-Manso1, Gloria Del Barrio1, Kevin P Dalton1, Lorenzo González-Molleda1, José Arrojo-Fernández1, Inés Nicieza1, Francisco Parra1.
Abstract
The mechanisms of calicivirus attachment and internalization are not well understood, mainly due to the lack of a reliable cell-culture system for most of its members. In this study, rabbit vesivirus (RaV) virions were shown to bind annexin A2 (ANXA2) in a membrane protein fraction from HEK293T cells, using a virus overlay protein-binding assay and matrix-assisted laser desorption/ionization time-of-flight analysis. A monoclonal anti-ANXA2 antibody and small interfering RNA-mediated knockdown of ANXA2 expression in HEK293T cells reduced virus infection significantly, further supporting the role of ANXA2 in RaV attachment and/or internalization.Entities:
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Year: 2009 PMID： 19605586 DOI： 10.1099/vir.0.013276-0
Source DB: PubMed Journal: J Gen Virol ISSN： 0022-1317 Impact factor: 3.891