Literature DB >> 1960422

A simple method for the purification of human peripheral blood monocytes. A substitute for Sepracell-MN.

E M Denholm1, F M Wolber.   

Abstract

Sepracell-MN has provided a simple, rapid means of isolating peripheral blood monocytes. However this product is no longer available. Consequentially we have developed a Percoll gradient which matches Sepracell-MN in simplicity and yield of monocytes. Using this Percoll gradient, an average of 7 x 10(6) monocytes with a purity of 83% were obtained from 30-40 ml of blood. These monocytes were at least 97% viable and responded to chemotactic stimuli in comparable numbers to those prepared using Sepracell-MN.

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Year:  1991        PMID： 1960422     DOI： 10.1016/0022-1759(91)90092-t

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  20 in total

1.  Infection of primary human monocytes by Epstein-Barr virus.

Authors:  M Savard; C Bélanger; M Tardif; P Gourde; L Flamand; J Gosselin
Journal:  J Virol       Date:  2000-03       Impact factor: 5.103

2.  Qualitative and quantitative analysis of monocyte transendothelial migration by confocal microscopy and three-dimensional image reconstruction.

Authors:  A V Andjelkovic; M R Zochowski; F Morgan; J S Pachter
Journal:  In Vitro Cell Dev Biol Anim       Date:  2001-02       Impact factor: 2.416

3.  Continuous secretion of monocyte chemotactic factors and fibroblast growth factors by alveolar macrophages following a single exposure to bleomycin in vitro.

Authors:  E M Denholm
Journal:  Am J Pathol       Date:  1992-10       Impact factor: 4.307

4.  Selective killing of human monocytes and cytokine release provoked by sphingomyelinase (beta-toxin) of Staphylococcus aureus.

Authors:  I Walev; U Weller; S Strauch; T Foster; S Bhakdi
Journal:  Infect Immun       Date:  1996-08       Impact factor: 3.441

5.  Pore-forming toxins trigger shedding of receptors for interleukin 6 and lipopolysaccharide.

Authors:  I Walev; P Vollmer; M Palmer; S Bhakdi; S Rose-John
Journal:  Proc Natl Acad Sci U S A       Date:  1996-07-23       Impact factor: 11.205

6.  TIF1beta functions as a coactivator for C/EBPbeta and is required for induced differentiation in the myelomonocytic cell line U937.

Authors:  J W Rooney; K L Calame
Journal:  Genes Dev       Date:  2001-11-15       Impact factor: 11.361

7.  Decreased cystathionine-γ-lyase (CSE) activity in livers of type 1 diabetic rats and peripheral blood mononuclear cells (PBMC) of type 1 diabetic patients.

Authors:  Prasenjit Manna; Neslihan Gungor; Robert McVie; Sushil K Jain
Journal:  J Biol Chem       Date:  2014-03-07       Impact factor: 5.157

8.  Collection, Storage, and Preparation of Human Blood Cells.

Authors:  Pradeep K Dagur; J Philip McCoy
Journal:  Curr Protoc Cytom       Date:  2015-07-01

9.  The down-regulation of cathepsin G in THP-1 monocytes after infection with Mycobacterium tuberculosis is associated with increased intracellular survival of bacilli.

Authors:  Carlos A Rivera-Marrero; Julie Stewart; William M Shafer; Jesse Roman
Journal:  Infect Immun       Date:  2004-10       Impact factor: 3.441

10.  Novel aspect of amphotericin B action: accumulation in human monocytes potentiates killing of phagocytosed Candida albicans.

Authors:  E Martin; A Stüben; A Görz; U Weller; S Bhakdi
Journal:  Antimicrob Agents Chemother       Date:  1994-01       Impact factor: 5.191
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