Fisnik Kurshumliu1, Christoph Thorns2, Lumturije Gashi-Luci3. 1. Institute of Anatomic Pathology, Medical School, University of Prishtina, Prishtina, Kosovo. Electronic address: fkurshumliu@gmail.com. 2. Institute of Pathology and German Reference and Consultation Centre for Haematopathology and Lymphoproliferative Diseases, University Clinic Schleswig-Holstein, Campus Luebeck, Germany. 3. Institute of Anatomic Pathology, Medical School, University of Prishtina, Prishtina, Kosovo.
Abstract
OBJECTIVE: The aim of this study was to validate the role of p16(INK4a) immunocytochemistry as a marker of cervical epithelial neoplasia in cytology and biopsy samples. METHODS: During the period of 4 years a total of 501 immunocytochemistry stains in cytology and biopsy samples were performed. The cytology smears were obtained from two independent laboratories of women with low-grade squamous intraepithelial lesion interpretation of Pap test or ThinPrep. Additionally, a selection of paraffin blocks from cases diagnosed previously with squamous intraepithelial lesion and cervical cancer were immunostained. RESULTS: Among 312 cytology samples analyzed by immunocytochemistry, 36.2% were positive for p16(INK4a). Negative cases were followed-up with conventional Pap smear and 86.9% showed regression. Among positive patients, 14 underwent surgery and 100% exhibited squamous intraepithelial lesion. In additional immunohistochemical analysis of selected biopsies from our archive, we found that the ratio of positivity for p16(INK4a) increased from 40% in L-SIL/CIN1 to 69.8% in H-SIL/CIN2 to 100% in H-SIL/CIN3 and squamous cell carcinoma. A relatively low number (24%) of cases with squamous metaplasia revealed focal and/or sporadic staining in the superficial layers of the epithelium. All the cases of cervical adenocarcinoma and mesonephric carcinoma were positive for p16(INK4a). CONCLUSION: p16(INK4a) is a useful additional marker for interpretation of problematic Pap smears and reduces the variability during evaluation of suspicious biopsies of the uterine cervix.
OBJECTIVE: The aim of this study was to validate the role of p16(INK4a) immunocytochemistry as a marker of cervical epithelial neoplasia in cytology and biopsy samples. METHODS: During the period of 4 years a total of 501 immunocytochemistry stains in cytology and biopsy samples were performed. The cytology smears were obtained from two independent laboratories of women with low-grade squamous intraepithelial lesion interpretation of Pap test or ThinPrep. Additionally, a selection of paraffin blocks from cases diagnosed previously with squamous intraepithelial lesion and cervical cancer were immunostained. RESULTS: Among 312 cytology samples analyzed by immunocytochemistry, 36.2% were positive for p16(INK4a). Negative cases were followed-up with conventional Pap smear and 86.9% showed regression. Among positive patients, 14 underwent surgery and 100% exhibited squamous intraepithelial lesion. In additional immunohistochemical analysis of selected biopsies from our archive, we found that the ratio of positivity for p16(INK4a) increased from 40% in L-SIL/CIN1 to 69.8% in H-SIL/CIN2 to 100% in H-SIL/CIN3 and squamous cell carcinoma. A relatively low number (24%) of cases with squamous metaplasia revealed focal and/or sporadic staining in the superficial layers of the epithelium. All the cases of cervical adenocarcinoma and mesonephric carcinoma were positive for p16(INK4a). CONCLUSION:p16(INK4a) is a useful additional marker for interpretation of problematic Pap smears and reduces the variability during evaluation of suspicious biopsies of the uterine cervix.
Authors: Pavel Idelevich; Adi Elkeles; Elimelech Okon; Don Kristt; Dov Terkieltaub; Ilia Rivkin; Ilan Bruchim; Ami Fishman Journal: Diagn Pathol Date: 2010-11-11 Impact factor: 2.644
Authors: Enoc M Cortés-Malagón; José Bonilla-Delgado; José Díaz-Chávez; Alfredo Hidalgo-Miranda; Sandra Romero-Cordoba; Aykut Uren; Haydar Celik; Matthew McCormick; José A Munguía-Moreno; Eloisa Ibarra-Sierra; Jaime Escobar-Herrera; Paul F Lambert; Daniel Mendoza-Villanueva; Rosa M Bermudez-Cruz; Patricio Gariglio Journal: Virology Date: 2013-09-27 Impact factor: 3.616
Authors: Adrya Lúcia Peres; Keilla Maria Paz E Silva; Rosângela Ferreira Frade de Araújo; José Luiz de Lima Filho; Mário Ribeiro de Melo Júnior; Danyelly Bruneska Gondim Martins; Nicodemos Teles de Pontes Filho Journal: J Biomed Sci Date: 2016-05-12 Impact factor: 8.410