Literature DB >> 19594456

A device for automated hydrodynamic shearing of genomic DNA.

Aric Joneja1, Xiaohua Huang.   

Abstract

We describe a device for automated fragmentation of genomic DNA by hydrodynamic shearing using a filter screen with uniform pores. Human genomic DNA can be fragmented reproducibly to a range of 2000-12,000 bp by using various fluid flow rates and screens with 0.5-10-microm pores. The utilization of disposable screens eliminates sample cross-contamination and the tendency of device clogging, commonly encountered in single-orifice shearing devices.

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Year:  2009        PMID： 19594456      PMCID： PMC2880395          DOI： 10.2144/000113123

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  15 in total

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Authors:  Bruce A Roe
Journal:  Methods Mol Biol       Date:  2004

2.  Sequencing complete mitochondrial and plastid genomes.

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Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

3.  Rapid isolation of nuclei from carrot suspension culture cells using a BioNebulizer.

Authors:  C M Okpodu; D Robertson; W F Boss; R K Togasaki; S J Surzycki
Journal:  Biotechniques       Date:  1994-01       Impact factor: 1.993

4.  Partial CviJI digestion as an alternative approach to generate cosmid sublibraries for large-scale sequencing projects.

Authors:  J C Gingrich; D M Boehrer; S B Basu
Journal:  Biotechniques       Date:  1996-07       Impact factor: 1.993

5.  Control of partial digestion combining the enzymes dam methylase and MboI.

Authors:  J D Hoheisel; D Nizetic; H Lehrach
Journal:  Nucleic Acids Res       Date:  1989-12-11       Impact factor: 16.971

6.  A bacterial artificial chromosome library for sequencing the complete human genome.

Authors:  K Osoegawa; A G Mammoser; C Wu; E Frengen; C Zeng; J J Catanese; P J de Jong
Journal:  Genome Res       Date:  2001-03       Impact factor: 9.043

7.  Shotgun DNA sequencing using cloned DNase I-generated fragments.

Authors:  S Anderson
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971

8.  Representation of DNA sequences in recombinant DNA libraries prepared by restriction enzyme partial digestion.

Authors:  B Seed; R C Parker; N Davidson
Journal:  Gene       Date:  1982-09       Impact factor: 3.688

Review 9.  Approaches to rapid DNA sequence analysis.

Authors:  P L Deininger
Journal:  Anal Biochem       Date:  1983-12       Impact factor: 3.365

10.  Effects of ultrasound on nucleic acid bases.

Authors:  J R McKee; C L Christman; W D O'Brien; S Y Wang
Journal:  Biochemistry       Date:  1977-10-18       Impact factor: 3.162
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  2 in total

1.  Linear nicking endonuclease-mediated strand-displacement DNA amplification.

Authors:  Aric Joneja; Xiaohua Huang
Journal:  Anal Biochem       Date:  2011-02-20       Impact factor: 3.365

2.  High Efficiency Hydrodynamic DNA Fragmentation in a Bubbling System.

Authors:  Lanhui Li; Mingliang Jin; Chenglong Sun; Xiaoxue Wang; Shuting Xie; Guofu Zhou; Albert van den Berg; Jan C T Eijkel; Lingling Shui
Journal:  Sci Rep       Date:  2017-01-18       Impact factor: 4.379
  2 in total

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