OBJECTIVE: Our objective was to determine whether polymorphisms in the promoter region of the interleukin-10 gene are more common in women with cervical insufficiency compared with controls. STUDY DESIGN: We conducted a case-control study. Histories and blood were obtained from 121 cases and 157 controls. DNA was extracted and purified by using Puregene kits. Samples were analyzed for the interleukin-10 -1082 G/A polymorphism and interleukin-10.G microsatellite in the interleukin-10 gene promoter region. RESULTS: The -1082 G/A polymorphism in the promoter region of the interleukin-10 gene occurred with similar frequency in cases and controls. The interleukin-10.G microsatellite contained 10 alleles (G6-G16). The G13 allele was present more frequently in cases (24.1%) compared with controls (14.6%) (P = .05). CONCLUSION: Interleukin-10 is known to down-regulate inflammation. The G13 allele in the interleukin-10.G microsatellite occurred more frequently in women with cervical insufficiency compared with controls, suggesting that alterations in inflammatory processes may play a role in cervical insufficiency.
OBJECTIVE: Our objective was to determine whether polymorphisms in the promoter region of the interleukin-10 gene are more common in women with cervical insufficiency compared with controls. STUDY DESIGN: We conducted a case-control study. Histories and blood were obtained from 121 cases and 157 controls. DNA was extracted and purified by using Puregene kits. Samples were analyzed for the interleukin-10-1082 G/A polymorphism and interleukin-10.G microsatellite in the interleukin-10 gene promoter region. RESULTS: The -1082 G/A polymorphism in the promoter region of the interleukin-10 gene occurred with similar frequency in cases and controls. The interleukin-10.G microsatellite contained 10 alleles (G6-G16). The G13 allele was present more frequently in cases (24.1%) compared with controls (14.6%) (P = .05). CONCLUSION:Interleukin-10 is known to down-regulate inflammation. The G13 allele in the interleukin-10.G microsatellite occurred more frequently in women with cervical insufficiency compared with controls, suggesting that alterations in inflammatory processes may play a role in cervical insufficiency.
Authors: Hope M Wolf; Roberto Romero; Jerome F Strauss; Sonia S Hassan; Shawn J Latendresse; Bradley T Webb; Adi L Tarca; Nardhy Gomez-Lopez; Chaur-Dong Hsu; Timothy P York Journal: BMJ Open Date: 2022-03-17 Impact factor: 2.692
Authors: Ludmila Volozonoka; Dmitrijs Rots; Inga Kempa; Anna Kornete; Dace Rezeberga; Linda Gailite; Anna Miskova Journal: PLoS One Date: 2020-03-26 Impact factor: 3.240