Literature DB >> 1959127

Preparation and handling of aequorin solutions for the measurement of cellular Ca2+.

O Shimomura1.   

Abstract

Main characteristics of the various types of aequorin presently available for measuring cellular Ca2+, i.e. heterogeneous aequorin, isoaequorins, recombinant aequorin, fluorescein-labeled aequorin and semi-synthetic aequorins, are summarized. Basic techniques of preparing and handling the solutions of those aequorins for measuring Ca2+, including such techniques as concentrating aequorin solutions, freeze-drying, changing buffer composition, and the regeneration of active aequorin, are described.

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Year:  1991        PMID： 1959127     DOI： 10.1016/0143-4160(91)90060-r

Source DB:  PubMed          Journal:  Cell Calcium        ISSN： 0143-4160            Impact factor:   6.817


  5 in total

Review 1.  Plant calcium signaling and monitoring: pros and cons and recent experimental approaches.

Authors:  C Plieth
Journal:  Protoplasma       Date:  2001       Impact factor: 3.356

2.  The relative rate of aequorin regeneration from apoaequorin and coelenterazine analogues.

Authors:  O Shimomura; Y Kishi; S Inouye
Journal:  Biochem J       Date:  1993-12-15       Impact factor: 3.857

3.  Determination of resting free calcium in barnacle muscle using modified aequorins, buffered calcium injections, and simultaneous image-intensified video microscopy.

Authors:  E B Ridgway; A M Gordon
Journal:  J Muscle Res Cell Motil       Date:  1995-10       Impact factor: 2.698

4.  Imaging calcium dynamics in living plants using semi-synthetic recombinant aequorins.

Authors:  M R Knight; N D Read; A K Campbell; A J Trewavas
Journal:  J Cell Biol       Date:  1993-04       Impact factor: 10.539

Review 5.  Calcium and Heart Failure: How Did We Get Here and Where Are We Going?

Authors:  Natthaphat Siri-Angkul; Behzad Dadfar; Riya Jaleel; Jazna Naushad; Jaseela Parambathazhath; Angelia A Doye; Lai-Hua Xie; Judith K Gwathmey
Journal:  Int J Mol Sci       Date:  2021-07-09       Impact factor: 6.208
  5 in total

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