Literature DB >> 19590825

Protease-resistant core form of Bacillus thuringiensis Cry1Ie: monomeric and oligomeric forms in solution.

Shuyuan Guo1, Yancai Zhang, Fuping Song, Jie Zhang, Dafang Huang.   

Abstract

Cry1Ie is encoded by cry1Ie, a gene that is silent in Bacillus thuringiensis strains but can be over-expressed in Escherichia coli. A protease-resistant core form of Cry1Ie with a molecular weight of approx. 55 kDa was found among the products of trypsin digestion. The oligomer and monomer of the protease-resistant core form were purified separately. The oligomer fraction contained a small quantity of dimer and a large amount of aggregates larger than tetramers. The 50% lethal concentrations (LC(50)) of the full-length Cry1Ie and both the monomer and oligomer fractions of the protease-resistant core form against Plutella xylostella were 14, 21 and 1400 microg/ml, respectively.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19590825     DOI： 10.1007/s10529-009-0078-2

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  4 in total

1.  Potential Prepore Trimer Formation by the Bacillus thuringiensis Mosquito-specific Toxin: MOLECULAR INSIGHTS INTO A CRITICAL PREREQUISITE OF MEMBRANE-BOUND MONOMERS.

Authors:  Wilaiwan Sriwimol; Aratee Aroonkesorn; Somsri Sakdee; Chalermpol Kanchanawarin; Takayuki Uchihashi; Toshio Ando; Chanan Angsuthanasombat
Journal:  J Biol Chem       Date:  2015-06-25       Impact factor: 5.157

2.  Identification of a New cry1I-Type Gene as a Candidate for Gene Pyramiding in Corn To Control Ostrinia Species Larvae.

Authors:  Can Zhao; Juan Luis Jurat-Fuentes; Heba M Abdelgaffar; Hongyu Pan; Fuping Song; Jie Zhang
Journal:  Appl Environ Microbiol       Date:  2015-03-20       Impact factor: 4.792

3.  A novel α-galactosidase from the thermophilic probiotic Bacillus coagulans with remarkable protease-resistance and high hydrolytic activity.

Authors:  Ruili Zhao; Rui Zhao; Yishuai Tu; Xiaoming Zhang; Liping Deng; Xiangdong Chen
Journal:  PLoS One       Date:  2018-05-08       Impact factor: 3.240

4.  Study of the Bacillus thuringiensis Cry1Ia Protein Oligomerization Promoted by Midgut Brush Border Membrane Vesicles of Lepidopteran and Coleopteran Insects, or Cultured Insect Cells.

Authors:  Ayda Khorramnejad; Mikel Domínguez-Arrizabalaga; Primitivo Caballero; Baltasar Escriche; Yolanda Bel
Journal:  Toxins (Basel)       Date:  2020-02-21       Impact factor: 4.546
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.