Zhou-Yan Feng1, Xiao-Jing Zheng, Jing Wang. 1. Key Lab of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310003, China. hnfzy@yahoo.com.cn
Abstract
OBJECTIVE: To directly examine the effects of carnosine on neuronal excitation and inhibition in rat hippocampus in vivo. METHODS: Artificial cerebrospinal fluid with carnosine was directly administrated over the exposed rat hippocampus. The changes of neuron activity in the CA1 region of hippocampus were evaluated by orthodromically- and antidromically-evoked potentials, as well as paired-pulse stimulation paradigm. RESULTS: In both orthodromic and antidromic response potentials, carnosine transformed population spikes (PSs) with single spike into epileptiform multiple spikes. In addition, similar to the effect of (-aminobutyric acid(A) (GABA(A)) antagonist picrotoxin, carnosine decreased paired-pulse stimulating depression significantly. However, no significant change was observed in the spontaneous field potentials during the application of carnosine. CONCLUSION: The results indicate a disinhibition-induced excitation effect of carnosine on the CA1 pyramidal neurons. It provides important information against the application of carnosine as a potential anticonvulsant in clinical treatment.
OBJECTIVE: To directly examine the effects of carnosine on neuronal excitation and inhibition in rat hippocampus in vivo. METHODS: Artificial cerebrospinal fluid with carnosine was directly administrated over the exposed rat hippocampus. The changes of neuron activity in the CA1 region of hippocampus were evaluated by orthodromically- and antidromically-evoked potentials, as well as paired-pulse stimulation paradigm. RESULTS: In both orthodromic and antidromic response potentials, carnosine transformed population spikes (PSs) with single spike into epileptiform multiple spikes. In addition, similar to the effect of (-aminobutyric acid(A) (GABA(A)) antagonist picrotoxin, carnosine decreased paired-pulse stimulating depression significantly. However, no significant change was observed in the spontaneous field potentials during the application of carnosine. CONCLUSION: The results indicate a disinhibition-induced excitation effect of carnosine on the CA1 pyramidal neurons. It provides important information against the application of carnosine as a potential anticonvulsant in clinical treatment.
Authors: Ivana Jukić; Nikolina Kolobarić; Ana Stupin; Anita Matić; Nataša Kozina; Zrinka Mihaljević; Martina Mihalj; Petar Šušnjara; Marko Stupin; Željka Breškić Ćurić; Kristina Selthofer-Relatić; Aleksandar Kibel; Anamarija Lukinac; Luka Kolar; Gordana Kralik; Zlata Kralik; Aleksandar Széchenyi; Marija Jozanović; Olivera Galović; Martina Medvidović-Kosanović; Ines Drenjančević Journal: Antioxidants (Basel) Date: 2021-06-28