Literature DB >> 19583979

Apolipoprotein E genotyping method by real time PCR, a fast and cost-effective alternative to the TaqMan and FRET assays.

Olga Calero1, Rafael Hortigüela, María J Bullido, Miguel Calero.   

Abstract

The apolipoprotein E gene (APOE) polymorphism genotyping has an allegedly important predictive value for coronary heart disorders and Alzheimer's disease. We developed a simple, fast, cost-effective and suited for high-throughput protocol for determining APOE genotypes by Real Time PCR monitored by SYBR Green. The method is based on differential amplification by allele-specific primers. These primers have variations in their 3'-end nucleotides such that are specific for one of the two variants in each polymorphic position. By this protocol, we obtained a 100% concordance with the APOE genotypes determined by sequencing analysis. The main advantages of this method are its relative simplicity and the reduced cost compared to other methodologies, such as the TaqMan and FRET assays.

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Year:  2009        PMID： 19583979     DOI： 10.1016/j.jneumeth.2009.06.033

Source DB:  PubMed          Journal:  J Neurosci Methods        ISSN： 0165-0270            Impact factor:   2.390


  36 in total

1.  Nephrotic syndrome associated with severe hypertriglyceridemia in a pediatric patient: Answers.

Authors:  Beatriz Corredor-Andrés; María Teresa Muñoz-Calvo; Olga Calero; Cristina Aparicio; Jesús Argente; Miguel Calero
Journal:  Pediatr Nephrol       Date:  2018-03-12       Impact factor: 3.714

2.  A novel mutation I215V in the PRNP gene associated with Creutzfeldt-Jakob and Alzheimer's diseases in three patients with divergent clinical phenotypes.

Authors:  Mercedes Muñoz-Nieto; Neus Ramonet; Juan Ignacio López-Gastón; Natividad Cuadrado-Corrales; Olga Calero; Marcos Díaz-Hurtado; José Ramón Ipiens; Santiago Ramón y Cajal; Jesús de Pedro-Cuesta; Miguel Calero
Journal:  J Neurol       Date:  2012-07-05       Impact factor: 4.849

3.  Apolipoprotein E ε4 Mediates Myelin Breakdown by Targeting Oligodendrocytes in Sporadic Alzheimer Disease.

Authors:  Gerald Wai-Yeung Cheng; Kingston King-Shi Mok; Sunny Hoi-Sang Yeung; Julia Kofler; Karl Herrup; Kai-Hei Tse
Journal:  J Neuropathol Exp Neurol       Date:  2022-08-16       Impact factor: 3.148

4.  Variability in APOE genotype status in human-derived cell lines: a cause for concern in cell culture studies?

Authors:  Sebastian Schaffer; Vanessa Y M Lam; Insa M A Ernst; Patricia Huebbe; Gerald Rimbach; Barry Halliwell
Journal:  Genes Nutr       Date:  2013-12-03       Impact factor: 5.523

5.  Argyrophilic Grain Disease: Demographics, Clinical, and Neuropathological Features From a Large Autopsy Study.

Authors:  Roberta Diehl Rodriguez; Claudia Kimie Suemoto; Mariana Molina; Camila Fernandes Nascimento; Renata Elaine Paraizo Leite; Renata Eloah de Lucena Ferretti-Rebustini; José Marcelo Farfel; Helmut Heinsen; Ricardo Nitrini; Kenji Ueda; Carlos Augusto Pasqualucci; Wilson Jacob-Filho; Kristine Yaffe; Lea Tenenholz Grinberg
Journal:  J Neuropathol Exp Neurol       Date:  2016-06-09       Impact factor: 3.685

6.  Diabetes is Not Associated with Alzheimer's Disease Neuropathology.

Authors:  Maria Niures Pimentel Dos Santos Matioli; Claudia Kimie Suemoto; Roberta Diehl Rodriguez; Daniela Souza Farias; Magnólia Moreira da Silva; Renata Elaine Paraizo Leite; Renata Eloah Lucena Ferretti-Rebustini; José Marcelo Farfel; Carlos Augusto Pasqualucci; Wilson Jacob Filho; Zoe Arvanitakis; Michel Satya Naslavsky; Mayana Zatz; Lea Tenenholz Grinberg; Ricardo Nitrini
Journal:  J Alzheimers Dis       Date:  2017       Impact factor: 4.472

7.  Human ApoE Isoforms Differentially Modulate Brain Glucose and Ketone Body Metabolism: Implications for Alzheimer's Disease Risk Reduction and Early Intervention.

Authors:  Long Wu; Xin Zhang; Liqin Zhao
Journal:  J Neurosci       Date:  2018-07-02       Impact factor: 6.167

8.  Expression analysis, single nucleotide polymorphisms and combined genotypes in candidate genes and their associations with growth and carcass traits in Qinchuan cattle.

Authors:  Li-jun Wang; Xiao-lin Liu; Hong-liang Wang; Hua He; Zhi-xiong Li; Ling Chen
Journal:  Mol Biol Rep       Date:  2012-11-30       Impact factor: 2.316

9.  African ancestry protects against Alzheimer's disease-related neuropathology.

Authors:  D Schlesinger; L T Grinberg; J G Alba; M S Naslavsky; L Licinio; J M Farfel; C K Suemoto; R E de Lucena Ferretti; R E P Leite; M P de Andrade; A C F dos Santos; H Brentani; C A Pasqualucci; R Nitrini; W Jacob-Filho; M Zatz
Journal:  Mol Psychiatry       Date:  2011-11-08       Impact factor: 15.992

10.  Genetic cross-interaction between APOE and PRNP in sporadic Alzheimer's and Creutzfeldt-Jakob diseases.

Authors:  Olga Calero; María J Bullido; Jordi Clarimón; Ana Frank-García; Pablo Martínez-Martín; Alberto Lleó; María Jesús Rey; Alberto Rábano; Rafael Blesa; Teresa Gómez-Isla; Fernando Valdivieso; Jesús de Pedro-Cuesta; Isidro Ferrer; Miguel Calero
Journal:  PLoS One       Date:  2011-07-20       Impact factor: 3.240
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