Literature DB >> 19579659

The rapid detection of Salmonella from food samples by loop-mediated isothermal amplification (LAMP).

Shigeko Ueda1, Yoshihiro Kuwabara.   

Abstract

Loop-mediated isothermal amplification (LAMP) assay was applied to the detection of Salmonella in food and human materials. It was possible for the assay to detect Salmonella within 60 min. All of 54 serovars of Salmonella tested were amplified, but all bacteria tested other than Salmonella were not. The LAMP assay could detect 10(2) cfu/ml levels of Salmonella. The specificity was similar to that of a PCR assay, but the sensitivity of LAMP was considered to be greater. Thus, the LAMP assay was confirmed to be a rapid, specific and sensitive detection method for Salmonella.

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Year:  2009        PMID： 19579659     DOI： 10.4265/bio.14.73

Source DB:  PubMed          Journal:  Biocontrol Sci        ISSN： 1342-4815            Impact factor:   0.982


  9 in total

1.  Rapid and simple detection of methicillin-resistance Staphylococcus aureus by orfX loop-mediated isothermal amplification assay.

Authors:  Jianyu Su; Xiaochen Liu; Hemiao Cui; Yanyan Li; Dingqiang Chen; Yanmei Li; Guangchao Yu
Journal:  BMC Biotechnol       Date:  2014-01-24       Impact factor: 2.563

2.  Loop-mediated isothermal amplification assays for screening of bacterial integrons.

Authors:  Guangchao Yu; Lei Chen; Chii-wann Lin; Bing Li; Hemiao Cui; Siyi Chen; Jian Miao; Huawei Bian; Dingqiang Chen; Yang Deng
Journal:  Biol Res       Date:  2014-10-02       Impact factor: 5.612

3.  Rapid and Sensitive Detection of Shigella spp. and Salmonella spp. by Multiple Endonuclease Restriction Real-Time Loop-Mediated Isothermal Amplification Technique.

Authors:  Yi Wang; Yan Wang; Lijuan Luo; Dongxin Liu; Xia Luo; Yanmei Xu; Shoukui Hu; Lina Niu; Jianguo Xu; Changyun Ye
Journal:  Front Microbiol       Date:  2015-12-14       Impact factor: 5.640

Review 4.  Detecting amplicons of loop-mediated isothermal amplification.

Authors:  Kazuya Shirato
Journal:  Microbiol Immunol       Date:  2019-08-13       Impact factor: 1.955

5.  Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP).

Authors:  Kazuya Shirato; Takuya Yano; Syouhei Senba; Shigehiro Akachi; Takashi Kobayashi; Takamichi Nishinaka; Tsugunori Notomi; Shutoku Matsuyama
Journal:  Virol J       Date:  2014-08-08       Impact factor: 4.099

6.  Rapid detection of Salmonella Typhi by loop-mediated isothermal amplification (LAMP) method.

Authors:  J Abdullah; N Saffie; F A R Sjasri; A Husin; Z Abdul-Rahman; A Ismail; I Aziah; M Mohamed
Journal:  Braz J Microbiol       Date:  2015-03-04       Impact factor: 2.476

7.  Development and application of loop-mediated isothermal amplification assays for rapid visual detection of cry2Ab and cry3A genes in genetically-modified crops.

Authors:  Feiwu Li; Wei Yan; Likun Long; Xing Qi; Congcong Li; Shihong Zhang
Journal:  Int J Mol Sci       Date:  2014-08-27       Impact factor: 5.923

Review 8.  Loop-Mediated Isothermal Amplification for Salmonella Detection in Food and Feed: Current Applications and Future Directions.

Authors:  Qianru Yang; Kelly J Domesle; Beilei Ge
Journal:  Foodborne Pathog Dis       Date:  2018-06       Impact factor: 3.171

9.  Development of fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) using quenching probes for the detection of the Middle East respiratory syndrome coronavirus.

Authors:  Kazuya Shirato; Shohei Semba; Sherif A El-Kafrawy; Ahmed M Hassan; Ahmed M Tolah; Ikuyo Takayama; Tsutomu Kageyama; Tsugunori Notomi; Wataru Kamitani; Shutoku Matsuyama; Esam Ibraheem Azhar
Journal:  J Virol Methods       Date:  2018-05-12       Impact factor: 2.014
  9 in total

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