Literature DB >> 19579008

Purification of Schwann cells from adult rats by differential detachment.

Wei Wu1, Yu-Qing Jin, James D Kretlow, Liang Xu, Hui-Chuan Duan, Zuo-Liang Qi.   

Abstract

Differential detachment by collagenase treatment is a new and efficient method for Schwann cell (SC) purification. As its effect on adult animals remains unclear, we have investigated the possibility of SC purification from adult rats. To avoid any systematic bias, Schwann cell purity before and after purification were compared by morphology, immunostaining of P75(NTR) and S100 and flow cytometric analysis. The final SC purities reached 99% as confirmed by three independent analyses SC purity and the cell yields were above 10(6) cells after two rounds of purification. The method of differential detachment is also suitable for SC purification in adult rats and could be useful for research and clinical applications.

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Year:  2009        PMID： 19579008     DOI： 10.1007/s10529-009-0064-8

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  8 in total

1.  A Simplified Protocol for the Purification of Schwann Cells and Exosome Isolation from C57BL/6 Mice.

Authors:  Mana Shojapour; Ghasem Mosayebi; Reza Hajihossein; Farshid Noorbakhsh; Aram Mokarizadeh; Mohammad Hossein Ghahremani
Journal:  Rep Biochem Mol Biol       Date:  2018-10

2.  Sciatic nerve regeneration in rats by a promising electrospun collagen/poly(ε-caprolactone) nerve conduit with tailored degradation rate.

Authors:  Wenwen Yu; Wen Zhao; Chao Zhu; Xiuli Zhang; Dongxia Ye; Wenjie Zhang; Yong Zhou; Xinquan Jiang; Zhiyuan Zhang
Journal:  BMC Neurosci       Date:  2011-07-15       Impact factor: 3.288

3.  Dispase rapidly and effectively purifies Schwann cells from newborn mice and adult rats.

Authors:  Jiaxue Zhu; Jinbao Qin; Zunli Shen; James D Kretlow; Xiaopan Wang; Zhangyin Liu; Yuqing Jin
Journal:  Neural Regen Res       Date:  2012-02-05       Impact factor: 5.135

4.  Specific marker expression and cell state of Schwann cells during culture in vitro.

Authors:  Zhangyin Liu; Yu-Qing Jin; Lulu Chen; Yang Wang; Xiaonan Yang; Jia Cheng; Wei Wu; Zuoliang Qi; Zunli Shen
Journal:  PLoS One       Date:  2015-04-10       Impact factor: 3.240

5.  The effect of co-transplantation of nerve fibroblasts and Schwann cells on peripheral nerve repair.

Authors:  Yang Wang; Dong Li; Gangyang Wang; Lulu Chen; Jun Chen; Zhangyin Liu; Zhaofeng Zhang; Hua Shen; Yuqing Jin; Zunli Shen
Journal:  Int J Biol Sci       Date:  2017-11-02       Impact factor: 6.580

6.  Direct Conversion of Human Fibroblasts into Schwann Cells that Facilitate Regeneration of Injured Peripheral Nerve In Vivo.

Authors:  Yoshihiro Sowa; Tsunao Kishida; Koichi Tomita; Kenta Yamamoto; Toshiaki Numajiri; Osam Mazda
Journal:  Stem Cells Transl Med       Date:  2017-01-09       Impact factor: 6.940

7.  Automated image analysis of stained cytospins to quantify Schwann cell purity and proliferation.

Authors:  Tamara Weiss; Lorenz Semmler; Flavia Millesi; Anda Mann; Maximilian Haertinger; Manuel Salzmann; Christine Radtke
Journal:  PLoS One       Date:  2020-05-22       Impact factor: 3.240

8.  Programming of Regulatory T Cells In Situ for Nerve Regeneration and Long-Term Patency of Vascular Grafts.

Authors:  Yanhong Wang; Fangchao Xue; Yanzhao Li; Lin Lin; Yeqin Wang; Shanlan Zhao; Xingli Zhao; Yong Liu; Ju Tan; Gang Li; Haoran Xiao; Juan Yan; Hao Tian; Min Liu; Qiao Zhang; Zhaojing Ba; Lang He; Wenyan Zhao; Chuhong Zhu; Wen Zeng
Journal:  Research (Wash D C)       Date:  2022-07-19
  8 in total

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