Literature DB >> 19575479

Laser microdissection-mediated isolation and in vitro transcriptional amplification of plant RNA.

Michael J Scanlon1, Kazuhiro Ohtsu, Marja C P Timmermans, Patrick S Schnable.   

Abstract

Protocols for laser microdissection and linear amplification of RNA from fixed, sectioned plant tissues are described. When combined with quantitative RT-PCR, microarray analysis, or RNA-sequencing, these procedures enable quantitative analyses of transcript accumulation from microscopic quantities of specific plant organs, tissues, or single cells.

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Year:  2009        PMID： 19575479     DOI： 10.1002/0471142727.mb25a03s87

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


  10 in total

1.  Single prokaryotic cell isolation and total transcript amplification protocol for transcriptomic analysis.

Authors:  Yun Kang; Ian McMillan; Michael H Norris; Tung T Hoang
Journal:  Nat Protoc       Date:  2015-06-04       Impact factor: 13.491

Review 2.  Laser assisted microdissection, an efficient technique to understand tissue specific gene expression patterns and functional genomics in plants.

Authors:  Vibhav Gautam; Ananda K Sarkar
Journal:  Mol Biotechnol       Date:  2015-04       Impact factor: 2.695

3.  Transcript amplification from single bacterium for transcriptome analysis.

Authors:  Yun Kang; Michael H Norris; Jan Zarzycki-Siek; William C Nierman; Stuart P Donachie; Tung T Hoang
Journal:  Genome Res       Date:  2011-05-02       Impact factor: 9.043

4.  Ontogeny of the maize shoot apical meristem.

Authors:  Elizabeth M Takacs; Jie Li; Chuanlong Du; Lalit Ponnala; Diane Janick-Buckner; Jianming Yu; Gary J Muehlbauer; Patrick S Schnable; Marja C P Timmermans; Qi Sun; Dan Nettleton; Michael J Scanlon
Journal:  Plant Cell       Date:  2012-08-21       Impact factor: 11.277

5.  Genome-wide transcriptome analysis of gametophyte development in Physcomitrella patens.

Authors:  Lihong Xiao; Hui Wang; Ping Wan; Tingyun Kuang; Yikun He
Journal:  BMC Plant Biol       Date:  2011-12-15       Impact factor: 4.215

6.  An Efficient LCM-Based Method for Tissue Specific Expression Analysis of Genes and miRNAs.

Authors:  Vibhav Gautam; Archita Singh; Sharmila Singh; Ananda K Sarkar
Journal:  Sci Rep       Date:  2016-02-10       Impact factor: 4.379

7.  A high-resolution gene expression atlas links dedicated meristem genes to key architectural traits.

Authors:  Steffen Knauer; Marie Javelle; Lin Li; Xianran Li; Xiaoli Ma; Kokulapalan Wimalanathan; Sunita Kumari; Robyn Johnston; Samuel Leiboff; Robert Meeley; Patrick S Schnable; Doreen Ware; Carolyn Lawrence-Dill; Jianming Yu; Gary J Muehlbauer; Michael J Scanlon; Marja C P Timmermans
Journal:  Genome Res       Date:  2019-11-19       Impact factor: 9.043

8.  Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level.

Authors:  Zhen Ma; Qiuying Liu; Huaxiao Yang; Raymond B Runyan; Carol A Eisenberg; Meifeng Xu; Thomas K Borg; Roger Markwald; Yifei Wang; Bruce Z Gao
Journal:  Light Sci Appl       Date:  2013       Impact factor: 17.782

9.  Transcriptome analysis of Gossypium hirsutum flower buds infested by cotton boll weevil (Anthonomus grandis) larvae.

Authors:  Sinara Artico; Marcelo Ribeiro-Alves; Osmundo Brilhante Oliveira-Neto; Leonardo Lima Pepino de Macedo; Sylvia Silveira; Maria Fátima Grossi-de-Sa; Adriana Pinheiro Martinelli; Marcio Alves-Ferreira
Journal:  BMC Genomics       Date:  2014-10-04       Impact factor: 3.969

10.  Plant homeodomain proteins provide a mechanism for how leaves grow wide.

Authors:  Phillip A Conklin; Robyn Johnston; Brianne R Conlon; Rena Shimizu; Michael J Scanlon
Journal:  Development       Date:  2020-10-21       Impact factor: 6.862
  10 in total

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