Literature DB >> 19574637

Crystallization and preliminary crystallographic analysis of bifunctional gamma-glutamylcysteine synthetase-glutatione synthetase from Streptococcus agalactiae.

Yasunori Nakashima1, Hiroshi Nii, Blythe E Janowiak, Owen W Griffith, Takao Hibi.   

Abstract

gamma-Glutamylcysteine synthetase-glutathione synthetase (gammaGCS-GS) is a bifunctional enzyme that catalyzes two consecutive steps of ATP-dependent peptide formation in glutathione biosynthesis. Streptococcus agalactiae gammaGCS-GS is a target for the development of potential therapeutic agents. gammaGCS-GS was crystallized using the sitting-drop vapour-diffusion method. The crystals grew to dimensions of 0.3 x 0.2 x 0.2 mm under reducing conditions with 5 mM TCEP. X-ray data were collected to 2.8 A resolution from a tetragonal crystal that belonged to space group I4(1).

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Year:  2009        PMID： 19574637      PMCID： PMC2705632          DOI： 10.1107/S1744309109018636

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  16 in total

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Authors:  Blythe E Janowiak; Michael A Hayward; Francis C Peterson; Brian F Volkman; Owen W Griffith
Journal:  Biochemistry       Date:  2006-09-05       Impact factor: 3.162

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Journal:  Science       Date:  1984-04-06       Impact factor: 47.728

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Authors:  C Sherrill; R C Fahey
Journal:  J Bacteriol       Date:  1998-03       Impact factor: 3.490

5.  Glutathione synthesis in Streptococcus agalactiae. One protein accounts for gamma-glutamylcysteine synthetase and glutathione synthetase activities.

Authors:  Blythe E Janowiak; Owen W Griffith
Journal:  J Biol Chem       Date:  2005-01-10       Impact factor: 5.157

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Authors:  H Gushima; T Miya; K Murata; A Kimura
Journal:  J Appl Biochem       Date:  1983-06

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Journal:  J Biol Chem       Date:  1975-02-25       Impact factor: 5.157

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Authors:  R C Fahey; W C Brown; W B Adams; M B Worsham
Journal:  J Bacteriol       Date:  1978-03       Impact factor: 3.490

9.  Existence of two D-alanine:D-alanine ligases in Escherichia coli: cloning and sequencing of the ddlA gene and purification and characterization of the DdlA and DdlB enzymes.

Authors:  L E Zawadzke; T D Bugg; C T Walsh
Journal:  Biochemistry       Date:  1991-02-12       Impact factor: 3.162

10.  Phaser crystallographic software.

Authors:  Airlie J McCoy; Ralf W Grosse-Kunstleve; Paul D Adams; Martyn D Winn; Laurent C Storoni; Randy J Read
Journal:  J Appl Crystallogr       Date:  2007-07-13       Impact factor: 3.304
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