Literature DB >> 19569099

Analysis of hydrophilic metabolites in physiological fluids by HPLC-MS using a silica hydride-based stationary phase.

Joseph J Pesek1, Maria T Matyska, Joseph A Loo, Steven M Fischer, Theodore R Sana.   

Abstract

Aqueous normal-phase chromatography was used for the analysis of metabolites in human saliva and urine samples. The column was packed with a silica hydride type separation material. Several gradients were tested with different mobile phase additives in order to produce retention for amino acids, small organic acids, and carbohydrates. Detection was done by TOF MS. In some cases the relative concentration levels of various metabolites in human saliva were compared for normal patients and patients with pancreatic cancer or pancreatitis. The reproducibility of retention of individual metabolites in these complex matrices was tested for several compounds.

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Year:  2009        PMID： 19569099     DOI： 10.1002/jssc.200900270

Source DB:  PubMed          Journal:  J Sep Sci        ISSN： 1615-9306            Impact factor:   3.645


  10 in total

Review 1.  Metabolomics in drug target discovery.

Authors:  J D Rabinowitz; J G Purdy; L Vastag; T Shenk; E Koyuncu
Journal:  Cold Spring Harb Symp Quant Biol       Date:  2011-11-23

2.  Micafungin alters the amino acid, nucleic acid and central carbon metabolism of Candida albicans at subinhibitory concentrations: novel insights into mechanisms of action.

Authors:  Aspasia Katragkou; Michael Williams; Sandi Sternberg; Dennis Pantazatos; Emmanuel Roilides; Thomas J Walsh
Journal:  J Antimicrob Chemother       Date:  2017-03-01       Impact factor: 5.790

3.  Development of isotope labeling LC-MS for human salivary metabolomics and application to profiling metabolome changes associated with mild cognitive impairment.

Authors:  Jiamin Zheng; Roger A Dixon; Liang Li
Journal:  Anal Chem       Date:  2012-12-06       Impact factor: 6.986

4.  Aqueous normal phase liquid chromatography coupled with tandem time-of-flight quadrupole mass spectrometry for determination of zanamivir in human serum.

Authors:  Jing Ge; Fengmao Liu; Eric H Holmes; Gary K Ostrander; Qing X Li
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2012-08-21       Impact factor: 3.205

5.  Essential but Not Vulnerable: Indazole Sulfonamides Targeting Inosine Monophosphate Dehydrogenase as Potential Leads against Mycobacterium tuberculosis.

Authors:  Yumi Park; Angela Pacitto; Tracy Bayliss; Laura A T Cleghorn; Zhe Wang; Travis Hartman; Kriti Arora; Thomas R Ioerger; Jim Sacchettini; Menico Rizzi; Stefano Donini; Tom L Blundell; David B Ascher; Kyu Rhee; Ardala Breda; Nian Zhou; Veronique Dartois; Surendranadha Reddy Jonnala; Laura E Via; Valerie Mizrahi; Ola Epemolu; Laste Stojanovski; Fred Simeons; Maria Osuna-Cabello; Lucy Ellis; Claire J MacKenzie; Alasdair R C Smith; Susan H Davis; Dinakaran Murugesan; Kirsteen I Buchanan; Penelope A Turner; Margaret Huggett; Fabio Zuccotto; Maria Jose Rebollo-Lopez; Maria Jose Lafuente-Monasterio; Olalla Sanz; Gracia Santos Diaz; Joël Lelièvre; Lluis Ballell; Carolyn Selenski; Matthew Axtman; Sonja Ghidelli-Disse; Hannah Pflaumer; Markus Bösche; Gerard Drewes; Gail M Freiberg; Matthew D Kurnick; Myron Srikumaran; Dale J Kempf; Simon R Green; Peter C Ray; Kevin Read; Paul Wyatt; Clifton E Barry; Helena I Boshoff
Journal:  ACS Infect Dis       Date:  2016-10-17       Impact factor: 5.084

6.  Untargeted plasma metabolite profiling reveals the broad systemic consequences of xanthine oxidoreductase inactivation in mice.

Authors:  Qiuying Chen; Hyeong-Cheon Park; Michael S Goligorsky; Praveen Chander; Steven M Fischer; Steven S Gross
Journal:  PLoS One       Date:  2012-06-18       Impact factor: 3.240

7.  Metabolomics-Based Discovery of Small Molecule Biomarkers in Serum Associated with Dengue Virus Infections and Disease Outcomes.

Authors:  Natalia V Voge; Rushika Perera; Sebabrata Mahapatra; Lionel Gresh; Angel Balmaseda; María A Loroño-Pino; Amber S Hopf-Jannasch; John T Belisle; Eva Harris; Carol D Blair; Barry J Beaty
Journal:  PLoS Negl Trop Dis       Date:  2016-02-25

8.  Impact of CodY protein on metabolism, sporulation and virulence in Clostridioides difficile ribotype 027.

Authors:  Nadine Daou; Yuanguo Wang; Vladimir M Levdikov; Madhumitha Nandakumar; Jonathan Livny; Laurent Bouillaut; Elena Blagova; Keshan Zhang; Boris R Belitsky; Kyu Rhee; Anthony J Wilkinson; Xingmin Sun; Abraham L Sonenshein
Journal:  PLoS One       Date:  2019-01-30       Impact factor: 3.240

9.  Global mass spectrometry based metabolomics profiling of erythrocytes infected with Plasmodium falciparum.

Authors:  Theodore R Sana; D Benjamin Gordon; Steven M Fischer; Shane E Tichy; Norton Kitagawa; Cindy Lai; William L Gosnell; Sandra P Chang
Journal:  PLoS One       Date:  2013-04-09       Impact factor: 3.240

Review 10.  Analysis of Nucleosides and Nucleotides in Plants: An Update on Sample Preparation and LC-MS Techniques.

Authors:  Henryk Straube; Claus-Peter Witte; Marco Herde
Journal:  Cells       Date:  2021-03-20       Impact factor: 6.600
  10 in total

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